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https://hdl.handle.net/11499/56524
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DC Field | Value | Language |
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dc.contributor.author | Çil, N. | - |
dc.contributor.author | Fenkçi, İ.V. | - |
dc.contributor.author | Mete, G.A. | - |
dc.contributor.author | Mutlu, D. | - |
dc.contributor.author | Kabukçu, C. | - |
dc.contributor.author | Çabuş, Ü. | - |
dc.date.accessioned | 2024-01-30T14:31:10Z | - |
dc.date.available | 2024-01-30T14:31:10Z | - |
dc.date.issued | 2024 | - |
dc.identifier.issn | 1309-9833 | - |
dc.identifier.uri | https://doi.org/10.31362/patd.1346146 | - |
dc.identifier.uri | https://hdl.handle.net/11499/56524 | - |
dc.description.abstract | Purposoe: Cell surface proteins known as Siglecs exhibit a specific affinity for sialic acid. Primarily located on the surface of immune cells, Siglecs belong to the subset of lectins called I-type lectins. Siglecs have important roles in maternal-fetal immune tolerance. We aimed to analyze the expression of Siglecs on Human Villous Trophoblasts (HVT) cells. Materials and methods: Total RNA was extracted from the HVT cell line, cDNA was synthesized, and real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) was performed the determine the changes in Siglec-3,-5,-6,-7,-8,-9,-10,-11, and-16 mRNA levels. In addition, Siglec levels were assessed by using immunohistochemical staining. Immunoreactivity against Siglec-6 and Siglec-9 was evaluated separately according to the intensity of brown color. Results: Expression levels of Siglec genes by qRT-PCR and melting curve analyses were performed using RNA extracted from the HVT cell line. Siglec-3,-5,-6,-7,-9,-10,-11, and-16 genes were found to be expressed in the HVT cell line. Differently, Siglec-8 results were undetected after cycle 40, which was considered a negative result. The immunocytochemical examination of the HVT cell line revealed that Siglec-6 expression was moderate in the cytoplasm (Score:2). Siglec-9 expression was prominent in the cytoplasm (Score:3). Conclusion: The results showed that Siglec-6 and Siglec-9 were expressed more than other Siglec proteins in the human trophoblast cells. Immunocytochemistry results also support these findings. Our study is the first to show Siglec-9 expression in human trophoblast cells. © 2024, Pamukkale University. All rights reserved. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Pamukkale University | en_US |
dc.relation.ispartof | Pamukkale Medical Journal | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.subject | human villous trophoblasts | en_US |
dc.subject | sialic acid | en_US |
dc.subject | Siglecs | en_US |
dc.title | Expression of Sialic Acid Binding Receptors (Siglecs) in human trophoblast cell line | en_US |
dc.title.alternative | İnsan Trofoblast Hücre Hattında Sialik Asit Bağlayıcı Reseptörlerin (Siglecs) Ekspresyonu | en_US |
dc.type | Article | en_US |
dc.identifier.volume | 17 | en_US |
dc.identifier.issue | 2 | en_US |
dc.department | Pamukkale University | en_US |
dc.identifier.doi | 10.31362/patd.1346146 | - |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.authorscopusid | 55392501500 | - |
dc.authorscopusid | 6507701460 | - |
dc.authorscopusid | 6507766696 | - |
dc.authorscopusid | 57212511655 | - |
dc.authorscopusid | 6506758719 | - |
dc.authorscopusid | 56690382800 | - |
dc.identifier.scopus | 2-s2.0-85180653512 | en_US |
dc.institutionauthor | … | - |
item.languageiso639-1 | en | - |
item.openairetype | Article | - |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.fulltext | With Fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
crisitem.author.dept | 14.03. Basic Medical Sciences | - |
crisitem.author.dept | 14.01. Surgical Medicine | - |
crisitem.author.dept | 14.03. Basic Medical Sciences | - |
crisitem.author.dept | 14.01. Surgical Medicine | - |
crisitem.author.dept | 14.01. Surgical Medicine | - |
Appears in Collections: | Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection Tıp Fakültesi Koleksiyonu |
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File | Size | Format | |
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Expression-of-Sialic-Acid-Binding-Receptors-Siglecs-in-human-trophoblast-cell-linePamukkale-Medical-Journal.pdf | 1.43 MB | Adobe PDF | View/Open |
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