Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/56727
Title: Antioxidant, AChE inhibitory, and anticancer effects of Verbascum thapsus extract
Authors: Zhang, N.
Baran, A.
Valioglu, F.
Teng, L.
Atalar, M.N.
Keskin, C.
Wang, X.-X.
Hatipoğlu, Abdulkerim
Baran, Mehmet Fırat
Abdelsalam, Amine Hafis
Arslan, Şevki
Necip, Adem
Karadağ, Musa
Alma, Mehmet Hakkı
Eftekhari, Aziz
Beilerli, Aferin
Keywords: AChE inhibition
Anticancer
Bioassays
Mullein
Plant extract
4 hydroxybenzaldehyde
4 hydroxybenzoic acid
6 hydroxyflavone
7 hydroxyflavone
acetylcholinesterase
antimitotic agent
Acetylcholinesterase
Antioxidants
Benzothiazoles
Caco-2 Cells
antioxidant
HEK293 Cells
Humans
Methanol
Phytochemicals
Plant Extracts
butylated hydroxyanisole
Sulfonic Acids
Tandem Mass Spectrometry
Verbascum
ascorbic acid
aurantiin
baicalein
cinnamic acid
baicalin
biochanin A
butylcresol
caffeic acid
catechin
chlorogenic acid
cholinesterase inhibitor
chrysin
coumaric acid
coumarin
cynarine
kaemerol 3 glucoside
cytotoxic agent
ellagic acid
fisetin
gallic acid
hesperetin
hesperidin
plant extract
hyperin
isoquercetin
kaempferol
morin
naringenin
neohesperidin
oleuropein
para coumaric acid
polydatine
protocatechuic acid
protocatechuic ethyl ester
shikimic acid
quercetin
quercetin 3 dextro xyloside
quercimeritrin
resveratrol
rosmarinic acid
rutoside
trans ferulic acid
salicylic acid
scutellarin
silibinin
sinapic acid
syringic acid
tacrine
tamarixetin
taxifolin
trolox C
unclassified drug
vanillic acid
sulfonic acid derivative
Verbascum thapsus extract
2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid
acetylcholinesterase
antioxidant
benzothiazole derivative
methanol
cell viability
phytochemical
plant extract
ABTS radical scavenging assay
antineoplastic activity
antiproliferative activity
Article
Caco-2 cell line
cell proliferation
chemical composition
cholinesterase inhibition
controlled study
limit of detection
cytotoxicity
DPPH radical scavenging assay
drug isolation
HEK293 cell line
human
human cell
Verbascum
IC50
in vitro study
limit of quantitation
liquid chromatography-mass spectrometry
LNCaP cell line
MTT assay
multiple reaction monitoring
phytochemistry
Verbascum thapsus
chemistry
HEK293S cell line
Male
male
tandem mass spectrometry
Publisher: Cellular and Molecular Biology Association
Abstract: Verbascum thapsus (Mullein) is a medicinal plant used in folk medicine to treat various ailments. For this study, the biological functions of Verbascum thapsus (VT) methanol extract were determined in vitro. The plant's methanol extract was created through the maceration process. The phytochemical composition of plant extracts was investigated using liquid chromatography-electrospray ionization tandem mass spectrometry. The antioxidant capacity of the extract was determined using the 2,2-diphenyl-1-picrylhydrazil (DPPH radical) and 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS radical). Cell lines Caco-2 (human colorectal adenocarcinoma cells), LNCaP (Lymph Node Carcinoma of Prostate), and HEK293 (Human embryonic kidney 293 cells) were used to model colon, prostate, and non-cancerous cells. The cytotoxic activity of the plant extract on the proliferation of these cells was determined by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, a tetrazole) assay protocol. VT extract showed moderate DPPH and ABTS radical scavenging activities at 30 mg/ml concentration. With this, VT extract was determined to inhibit acetylcholinesterase (AChE) enzyme and had strong cytotoxic activity on cancerous cell lines. In addition, our findings clearly showed that the plant extract had greater cytotoxic activity on the viability of cancerous cells compared to non-cancerous (Human embryonic kidney cells; HEK293) cells. The current findings showed that V. thapsus might be a promising anti-cancer medication candidate for the treatment of human colorectal adenocarcinoma and colon cancer, as well as a good source antioxidans. © 2023 by the C.M.B. Association.
URI: https://doi.org/10.14715/cmb/2023.69.14.35
https://hdl.handle.net/11499/56727
ISSN: 0145-5680
Appears in Collections:Fen Fakültesi Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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