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https://hdl.handle.net/11499/57048
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DC Field | Value | Language |
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dc.contributor.author | Çoban, H.Ş. | - |
dc.contributor.author | Çil, N. | - |
dc.contributor.author | Önder, E. | - |
dc.contributor.author | Abban, Mete, G. | - |
dc.date.accessioned | 2024-05-06T16:25:46Z | - |
dc.date.available | 2024-05-06T16:25:46Z | - |
dc.date.issued | 2024 | - |
dc.identifier.issn | 0301-4851 | - |
dc.identifier.uri | https://doi.org/10.1007/s11033-024-09422-8 | - |
dc.identifier.uri | https://hdl.handle.net/11499/57048 | - |
dc.description.abstract | Background: Ovarian cancer is the leading cause of gynecological cancer deaths. One of the major challenges in treating ovarian cancer with chemotherapy is managing the resistance developed by cancer cells to drugs, while also minimizing the side effects caused by these agents In the present study, we aimed to examine the effects of a combination of alpha lipoic acid (ALA), with cisplatin and paclitaxel in ovarian cancer(OVCAR-3). Methods: The cytotoxic effects of ALA, cisplatin and paclitaxel on OVCAR-3 cells were determined. Four groups were formed: Control, ALA, Cisplatin + Paclitaxel, ALA + Cisplatin + Paclitaxel. The effects of single and combined therapy on cell migration, invasion and colony formation were analyzed. Changes in the expression of genes related to apoptosis, cell adhesion and cell cycle were analyzed with Real-time polymerase chain reaction(RT-PCR). The oxidative stress index and The Annexin V test were performed. Results: The reduction in rapamycin-insensitive companion of mTOR(RICTOR) expression in the ALA + Cisplatin + Paclitaxel group was found statistically significant(p < 0.05). The decrease in MMP-9 and − 11 expressions the ALA + Cisplatin + Paclitaxel group was statistically significant(p < 0.05). The lowest values for mitogen-activated protein kinase(MAPK) proteins were found in the ALA + Cisplatin + Paclitaxel group. No colony formation was observed in the Cisplatin + Paclitaxel and ALA + Cisplatin + Paclitaxel groups. The lowest wound healing at 24 h was seen in the ALA + Cisplatin + Paclitaxel group. Conclusions: This study is the first one to investigate the combined treatment of ALA, Cisplatin, Paclitaxel on OVCAR-3. While ALA alone was not effective, combined therapy with ALA, has been found to reduce cell invasion, especially wound healing in the first 24 h, along with tumor cell adhesion. © The Author(s), under exclusive licence to Springer Nature B.V. 2024. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Springer Science and Business Media B.V. | en_US |
dc.relation.ispartof | Molecular Biology Reports | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | Alpha lipoic acid; Cisplatin; Ovarian cancer; OVCAR-3; Paclitaxel | en_US |
dc.subject | antineoplastic agent; cisplatin; paclitaxel; thioctic acid; transcription factor; adenocarcinoma; apoptosis; drug resistance; female; genetics; human; ovary carcinoma; ovary tumor; pathology; tumor cell line; Adenocarcinoma; Antineoplastic Agents; Apoptosis; Carcinoma, Ovarian Epithelial; Cell Line, Tumor; Cisplatin; Drug Resistance, Neoplasm; Female; Humans; Ovarian Neoplasms; Paclitaxel; Thioctic Acid; Transcription Factors | en_US |
dc.title | Anti-cancer effects of alpha lipoic acid, cisplatin and paclitaxel combination in the OVCAR-3 ovarian adenocarcinoma cell line | en_US |
dc.type | Article | en_US |
dc.identifier.volume | 51 | en_US |
dc.identifier.issue | 1 | en_US |
dc.department | Pamukkale University | en_US |
dc.identifier.doi | 10.1007/s11033-024-09422-8 | - |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.authorscopusid | 58605380400 | - |
dc.authorscopusid | 55392501500 | - |
dc.authorscopusid | 58644051800 | - |
dc.authorscopusid | 57193456387 | - |
dc.identifier.pmid | 38578399 | en_US |
dc.identifier.scopus | 2-s2.0-85189613019 | en_US |
dc.identifier.wos | WOS:001197733900011 | en_US |
dc.institutionauthor | … | - |
item.grantfulltext | none | - |
item.openairetype | Article | - |
item.languageiso639-1 | en | - |
item.fulltext | No Fulltext | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
crisitem.author.dept | 14.03. Basic Medical Sciences | - |
crisitem.author.dept | 14.03. Basic Medical Sciences | - |
Appears in Collections: | PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection Tıp Fakültesi Koleksiyonu WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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