Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/5712
Title: Effects of the calcium channel blocker nifedipine on monosynaptic reflexes
Authors: Genc, O.
Tasci, N.
Demir, S.
Marangoz, C.
Keywords: Calcium channel blocker
Monosynaptic reflex
Nifedipine
calcium channel
calcium channel blocking agent
nifedipine
animal experiment
animal model
article
cat
controlled study
dose response
drug effect
monosynaptic reflex
nonhuman
spinal cord
Abstract: The effects of calcium channel blocker, nifedipine on spinal monosynaptic reflexes were investigated in spinal cats. Nifedipine was administrated-locally (10, 50, 100s µmol/l) and intraperitoneally (5; 10, 20 mg/kg). Adult cats (n=10), weighing 1.5-3 kg were anesthetized with ketamine (50 mg/kg, I.M.) and artificially ventilated. Animals were spinalized at C1 level. A laminectomy was performed in the lumbosacral region. The ventral and dorsal roots of segment L5 were isolated and a pouch of skin was formed at the site of the dissection, to allow the exposed tissues to be covered with liquid paraffin, which was kept at 38.5 ± 0.5°C with a heating pad. A polyethylene cannula was introduced into the left carotid artery to monitor blood pressure, which was kept above 100 mmHg. The dorsal root of segment L5 was placed on a silver-silver chloride wire electrode for stimulation through an isolating unit. The reflex potentials were recorded from the ipsilateral L5 ventral root, mounted on a silver-silver chloride wire electrode. Both systemic and local application of nifedipine produced a dose-dependent decrease in the amplitude of the monosynaptic reflexes (p < 0.05). Moreover, the latency of the monosynaptic reflexes was increased after administration of the drug (p < 0.005). These results show the presence of voltage-dependent calcium channels in the spinal cord and these channels may play an important role to regulate reflex respond.
URI: https://hdl.handle.net/11499/5712
ISSN: 1300-2996
Appears in Collections:Tıp Fakültesi Koleksiyonu

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