An experimental study on investigation of myokine responses to acute and chronic swimming exercise in mice

Abstract

Objective: Exercise is known to have many beneficial effects for the organism, especially the musculoskeletal, cardiovascular, respiratory and endocrine systems, depending on the type, intensity, duration and frequency. One of the mechanisms that mediate these beneficial effects is cytokines released from muscle called myokines. It is important to examine myokine levels in response to exercise to understand their roles in muscle growth / differentiation, metabolism, angiogenesis, neuronal stimulation. We aimed to investigate the time-dependent changes in the plasma levels of ciliary neurotrophic factor (CNTF), CXC ligand 1 (CXCL-1), cardiotrophin 1 (CT-1), oncostatin M (OSM), myostatin (MSTN) in response to acute-chronic swimming exercise in mice. Material and Methods: 70 adult, BALB/c male mice were divided into control, acute swimming (30 min) and chronic swimming (30 min/day, 5 days/week, 6 weeks) groups. Exercising mice were further divided into 3, in terms of the time passed (3, 24, 48 h) following the last exercise session. Mice swam in a water tank (68 cm x 44 cm x 38 cm) at constant temperature (30-32 °C). Plasma CNTF, CXCL-1, CT-1, OSM, MSTN levels were determined using commercial kits. Results: No statistically significant time-dependent alteration in plasma levels of CNTF, CXCL1, CT-1, OSM, MSTN was determined. Conclusion: The fact that there is no change in plasma myokine concentrations at 3, 24 and 48 h following exercises may not exclude the possibility that these myokines play role in exercise-induced adaptations. If different sample collection times had been chosen, possible changes in these myokines may have been detected. © 2024 by Türkiye Klinikleri.