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https://hdl.handle.net/11499/57474
Title: | Investigation of diabetes-related molecular changes in embryo-endometrium crosstalk | Authors: | Tan, S. Tan, S. Tokgün, O. Çetin, H. Tokgün, E. Özdamar, S. |
Keywords: | Calcium cAMP Diabetes Implantation Matrix metalloproteases Pregnancy calcium gamma interferon inducible protein 10 gelatinase A gelatinase B glutathione peroxidase metalloproteinase toll like receptor 4 matrix metalloproteinase microRNA TIMP1 protein, rat tissue inhibitor of metalloproteinase 1 animal experiment animal tissue Article cAMP signaling controlled study diabetes mellitus differential gene expression down regulation embryo endometrium enzyme linked immunosorbent assay female gene ontology glucose blood level hierarchical clustering histology immunohistochemistry implantation KEGG MAPK signaling microarray analysis nonhuman pregnancy rat real time polymerase chain reaction receptor cross-talk RNA isolation upregulation animal experimental diabetes mellitus genetics mammalian embryo metabolism nidation pregnancy in diabetics signal transduction Sprague Dawley rat Animals Diabetes Mellitus, Experimental Embryo Implantation Embryo, Mammalian Endometrium Female Matrix Metalloproteinases MicroRNAs Pregnancy Pregnancy in Diabetics Rats Rats, Sprague-Dawley Signal Transduction Tissue Inhibitor of Metalloproteinase-1 |
Publisher: | Elsevier B.V. | Abstract: | The primary aim of this study was to explore the impact of diabetes on matrix metalloproteases and tissue inhibitors, crucial factors for successful implantation, and to elucidate the molecular mechanisms that undergo changes in the endometrium and the embryo during diabetic pregnancies. In this investigation, we established a streptozotocin-induced diabetic pregnant rat model. Microarray analysis followed by RT-PCR was utilized to identify gene regions exhibiting expression alterations. Subsequently, we assessed the effects of MMPs and tissue inhibitors using ELISA and immunohistochemistry techniques, in addition to analyzing changes at the genetic level. Diabetes led to the upregulation of MMP3, MMP9, and MMP20 on the 6.5th day of pregnancy, while causing the downregulation of MMP3, MMP9, and MMP11 on the 8.5th day of pregnancy. TIMP1 expression was downregulated on the 8.5th day compared to the control group. No statistically significant differences were observed between the groups regarding other TIMP expressions. KEGG pathway analysis revealed that diabetes induced alterations in the expression of genes associated with certain microRNAs, as well as signaling pathways such as cAMP, calcium, BMP, p53, MAPK, PI3K-Akt, Jak-STAT, Hippo, Wnt, and TNF. Additionally, gene ontology analysis unveiled changes in membrane structures, extracellular matrix, signaling pathways, ion binding, protein binding, cell adhesion molecule binding, and receptor-ligand activity. This study serves as a valuable guide for investigating the mechanisms responsible for complications in diabetic pregnancies. By revealing the early-stage effects of diabetes, it offers insight into the development of new diagnostic and treatment approaches, ultimately contributing to improved patient care. © 2024 Elsevier B.V. | URI: | https://doi.org/10.1016/j.gene.2024.148557 https://hdl.handle.net/11499/57474 |
ISSN: | 0378-1119 |
Appears in Collections: | PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection Tıp Fakültesi Koleksiyonu WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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