Effect of quercetin on oxidative stress in 3T3-L1 mature and hypertrophic cells

Abstract

Purpose: The process of excessive or abnormal accumulation of fat in the body is called obesity, and its prevalence is increasing globally. The imbalance between antioxidants and free radicals, or oxidative stress, can be caused by or result from obesity. Flavonoids with antioxidant potential may help lower the increased oxidative stress associated with obesity. This study aimed to determine how quercetin affected oxidative stress in hypertrophied and mature 3T3-L1 adipocytes. Materials and methods: After differentiating, 3T3-L1 adipocytes were treated with insulin and a glucose-containing medium to become mature (10 days) and hypertrophic (18 days). The cells were subsequently incubated with 80 μM quercetin for 24 and 48 hours. ELISA was used to determine the levels of total antioxidant total oxidant capacity (TAS/TOS). Using Oil Red O staining, an accumulation of triglycerides in cells was examined. Results: The results showed that quercetin molecule only increased TAS level on oxidative stress in mature adipocytes (TAS; M-C: 649.37±1.38; M-Q80: 655.87±1.68 p=0.0001), whereas it exerted a prooxidative effect in hypertrophic adipocytes (OSI; H-C: 4.90±0.19; H-Q80: 6.20±0.039 p=0.0001). Conclusion: It is believed that the administration of quercetin at the appropriate dose and duration in different fat cell types is crucial for the antioxidant mechanism of action that produces numerous beneficial effects.