Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/57954
Title: Investigation of the Efficacy of Sericin in Experimental Knee Osteoarthritis Model in Rats through the TGF-Beta/Smad Pathway
Other Titles: Deneysel Diz Osteoartrit Modeli Geliştirilen Sıçanlarda Serisinin Etkinliğinin TGF-Beta/Smad Yolağı Üzerinden İncelenmesi
Authors: Gündoğdu, K.
Kiliç, Erkek, Ö.
Gündoğdu, G.
Sayin, D.
Abban, Mete, G.
Keywords: nee osteoarthritis
sericin
TGF-β1/smad pathway
connective tissue growth factor
eosin
hematoxylin
iodoacetic acid
ketamine
monocyte chemotactic protein 1
sericin
Smad protein
transforming growth factor beta
animal experiment
animal model
animal tissue
Article
articular cartilage
bone tissue
cartilage
cartilage matrix
cartilage thickness
chondrocyte
connective tissue
controlled study
drug efficacy
enzyme linked immunosorbent assay
female
histology
histopathology
immunofluorescence
immunohistochemistry
knee joint
knee osteoarthritis
mesenchymal stem cell
microscopy
nonhuman
protein expression
rat
subchondral bone
Publisher: Ataturk Universitesi
Abstract: This study was aimed to investigate the therapeutic effectiveness of sericin in rats with monosodium iodoacetate (MIA)-induced knee osteoarthritis (KOA), focusing on evaluating its effectiveness via the TGF-β/Smad pathway. The KOA model was established through the injection of MIA into the knee joint, and the rats were randomly allocated into three groups: group 1 (control), group 2 (KOA control), and group 3 (KOA+sericin). Sericin was administered intra-articularly to rats on days 1,7,14, and 21 (0.8 g/kg/mL, 50 µL). After 21 days, the rats were sacrificed, and serum samples were analyzed using the ELISA method to measure transforming growth faktör-Beta (TGF-β1), mother against decapentaplegic homolog 2 (Smad2), and connective tissue growth factor (CTGF) levels. Additionally, knee joint samples underwent histopathological evaluations with hematoxylin-eosin staining and immunohistochemical assessment using TGF-β1 and Smad2/3 antibodies. Serum TGF-β1 and CTGF levels were significantly increased in group 2 vs. group 1 (P < .05). A statistically significant decrease was observed in group 3 (P < .05). Serum Smad2 levels were not significantly different between groups. Histopathologically, group 2 showed a subchondral bone tissue, degeneration of the cartilage and deep fissures. On the other hand, group 3 showed reduced degeneration in chondrocyte cells, increased cartilage thickness, and a cartilage matrix that appeared close to normal were noted. Immunohistochemically, group 2 exhibited an increase in TGF-β1 and Smad expression, whereas group 3 decreased these expressions than group 2. Sericin demonstrates potential efficacy in the experimental KOA model in rats through the TGF-β1/Smad pathway. Consequently, sericin may emerge as a promising therapeutic agent for the treatment of KOA with further support from advanced clinical trials. © 2024, Ataturk Universitesi. All rights reserved.
URI: https://doi.org/10.17094/vetsci.1415498
https://hdl.handle.net/11499/57954
ISSN: 2822-3608
Appears in Collections:Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Tıp Fakültesi Koleksiyonu

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