Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/58648
Title: S-sulfocysteine's Toxic Effects on Ht-22 Cells Are Not Triggered by Glutamate Receptors, nor Do They Involve Apoptotic or Genotoxicity Mechanisms
Authors: Tekin, Volkan
Altintas, Fatih
Oymak, Burak
Unal, Egem Burcu
Tunc-Ata, Melek
Elmas, Levent
Kucukatay, Vural
Keywords: S-Sulphocysteine
Cytotoxicity
Ht-22 Cell
Apoptosis
Publisher: Springer
Abstract: S-Sulfocysteine (SSC) is a metabolite derived from the metabolism of sulfur-containing amino acids. It has been implicated in neurotoxicity observed in children with sulfite oxidase deficiency. The aim of our study was to confirm the neurotoxic effects of SSC using a mouse hippocampal cell line (HT-22) and to investigate the role of apoptosis in these effects, especially in terms of caspase-3 activation and genotoxicity. Based on the viability graph obtained following increasing concentrations of SSC, we determined the LC50 dose of SSC to be 125 mu M by probit analysis. The cytotoxic effects of SSC were not reversed by glutamate receptor blocker administration. However, SSC treatment did not induce caspase-3 activation or induce DNA damage. Our results showed that SSC has a cytotoxic effect on neurons like glutamate, but glutamate receptor blockers reversed glutamate-induced toxicity, while these blockers did not protect neurons from SSC toxicity. The absence of caspase-3 activation and DNA fragmentation, which are indicative of apoptosis, in SSC-induced cell death suggests that alternative cell death pathways, such as necrosis and oxytosis may be implicated. Further research is necessary to fully elucidate SSC-induced cell death. The aim of our study was to confirm the neurotoxic effects of SSC using a mouse hippocampal cell line (HT-22) and to investigate the role of apoptosis in these effects, especially in terms of caspase-3 activation and genotoxicity.
URI: https://doi.org/10.1007/s10616-024-00697-0
https://hdl.handle.net/11499/58648
ISSN: 0920-9069
1573-0778
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Tıp Fakültesi Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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