Development of Dispersive Liquid-Liquid Microextraction for Determination of Some Chlorophenols in Water Samples by Gas Chromatography-Mass Spectrometry

Abstract

A dispersive liquid-liquid microextraction (DLLME) procedure coupled with gas chromatography-ion trap mass spectrometric detection was developed for determination and preconcentration of some chlorophenols (CPs) from aqueous solutions. A Plackett-Burman experimental design was employed to evaluate the influence of those parameters to be controlled in DLLME. Under the optimum experimental conditions found from this statistical evaluation (sample volume, 10 mL; extraction solvent: chloroform, volume, 150 μL; disperser solvent: acetone, volume, 1000 μL; pH, 2; extraction time: 5 min.), the figures of merit of the proposed method were evaluated. A broad linear range (150–1200 μgL-1) was obtained with a good linearity (r2=0.999). The limits of detection varied between 0.010 and 0.226 μgL-1 for the CPs. Relative standard deviations obtained by extracting aqueous sample spiked at 150 μgL-1of each the CPs were almost 6.0 %. The relative recoveries of real water samples spiked with different concentrations of CPs were in the range of 60–113 %. The proposed DLLME method was successfully applied to the rapid and convenient determination of CPs in water samples. © 2011, Copyright Har Krishan Bhalla & Sons.