Determination of Mesotrione, Simazine and Atrazine by Rp- Hplc in Thermal Water, Sediment and Vegetable Samples

Abstract

This paper presents a simple, relatively inexpensive method for the detection of a group of commonly used herbicides such as mesotrione, simazine and atrazine in vegetables, thermal water and sediment samples by means of reversed phase-high performance liquid chromatography (RP-HPLC) with ultraviolet detection (DAD). The calibration curves were linear in the concentration range of 0.10-5.00 μg/mL. The limits of detection calculated at a signal-to-noise ratio of 3 were in the range of 0.03-0.10 μg/mL. The uncertainties of analytical methods were lower than 10 %. The method was validated by spiking recovery test of mesotrione, simazine and atrazine from the samples. Simazine and atrazine in the concentration range of 0.03-0.82 μg/g were detected in most of the vegetable samples, whereas mesotrione in the concentration of 2.40 and 4.96 μg/ mL was found only in thermal waters and 0.28 μg/g in a sediment sample. © 2012, Copyright Har Krishan Bhalla & Sons.