Chrysin Protects Neuronal Cells Against Carboplatin Exposure-Induced Apoptosis and Oxidative Damage

Abstract

Chemotherapy drugs such as carboplatin (Carb) are widely used to treat various cancers, including testicular, lung, and ovarian cancer. Although Carb primarily targets cancer cells, it can also damage healthy cells, including neuron, leading to potential adverse effects. Notably, some side effects of Carb therapy are associated with nerve cells and the nervous system. This study aimed to investigate the potential protective effects of chrysin (Chr) against Carb-induced oxidative toxicity in SH-SY5Y neuronal cells. The ameliorative effects of Chr on Carb-induced cellular toxicity were evaluated through cell viability assay, lipid peroxidation (LPO) analysis, TUNEL assay and immunocytochemistry staining. The results indicated that Chr mitigates Carb toxicity in SH-SY5Y cells through alleviating cell viability and reducing LPO levels. The study also demonstrated that Carb treatment caused apoptosis by causing DNA strand breaks while Chr treatment alleviated these effects. These findings suggest that Chr, may diminish the apoptotic effects of Carb in SHSY5Y cells and could provide insights into potential therapeutic strategies for mitigating cell damage caused by Carb. © 2025 Suleyman Demirel University. All rights reserved.