Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/5964
Title: Comparison of the indirect immunofluorescence assay performance of Bartonella henselae antigens obtained by co-cultivation in vero and HeLa cells
Authors: Ergin, Çağrı
Akkaya, Yüksel
Satilmiş, O.K.
Yilmaz, C.
Keywords: Bartonella henselae
Co-cultivation
HeLa
Immunofluorescence antibody method
Serology
Vero
bacterial antigen
diagnostic agent
animal
article
Cercopithecus
coculture
comparative study
fluorescent antibody technique
HeLa cell
human
immunology
methodology
standard
Vero cell
Animals
Antigens, Bacterial
Cercopithecus aethiops
Coculture Techniques
Fluorescent Antibody Technique, Indirect
HeLa Cells
Humans
Vero Cells
Abstract: The laboratory diagnosis of Bartonella henselae infection is mainly based on serological testing by indirect immunofluorescence assay (IFA). Cell line co-cultivation with B.henselae and agar derivated antigens are the two major procedures used for evaluation of arnti-Bartonella antibodies. Vero and Hep-2 cell lines are the most commonly used media for co-cultivation both in-house and commercial diagnostic kits production. However, HeLa cells which are easily supplied and grown, also can easily be infected by B.henselae. The aim of this study was to compare the performances of antigens obtained by co-cultivation of B.henselae ATCC 49882 (Houston-1) in Vero and HeLa Cells in IFA serology. Out of 381 sera samples, 127 (33.3%) were found positive and 195 (51.2%) were found negative by IFA performed by both cell line co-cultivations. The total agreement between the methods were found as 84.5% (322/381), and Cohen kappa value was calculated as 0.68 (strong, coherent). As a result, HeLa cells were found to be useful for the preparation of B.henselae antigens to be used in IFA for the serologic diagnosis of B.henselae infections. However different genotype strains and cross reactions with other infectious agents should be investigated by further studies before routine applications of HeLa cell co-cultivations procedure is established.
URI: https://hdl.handle.net/11499/5964
ISSN: 0374-9096
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Tıp Fakültesi Koleksiyonu
TR Dizin İndeksli Yayınlar Koleksiyonu / TR Dizin Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

Show full item record



CORE Recommender

SCOPUSTM   
Citations

2
checked on Oct 13, 2024

WEB OF SCIENCETM
Citations

2
checked on Oct 22, 2024

Page view(s)

56
checked on Aug 24, 2024

Google ScholarTM

Check





Items in GCRIS Repository are protected by copyright, with all rights reserved, unless otherwise indicated.