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https://hdl.handle.net/11499/5964
Title: | Comparison of the indirect immunofluorescence assay performance of Bartonella henselae antigens obtained by co-cultivation in vero and HeLa cells | Authors: | Ergin, Çağrı Akkaya, Yüksel Satilmiş, O.K. Yilmaz, C. |
Keywords: | Bartonella henselae Co-cultivation HeLa Immunofluorescence antibody method Serology Vero bacterial antigen diagnostic agent animal article Cercopithecus coculture comparative study fluorescent antibody technique HeLa cell human immunology methodology standard Vero cell Animals Antigens, Bacterial Cercopithecus aethiops Coculture Techniques Fluorescent Antibody Technique, Indirect HeLa Cells Humans Vero Cells |
Abstract: | The laboratory diagnosis of Bartonella henselae infection is mainly based on serological testing by indirect immunofluorescence assay (IFA). Cell line co-cultivation with B.henselae and agar derivated antigens are the two major procedures used for evaluation of arnti-Bartonella antibodies. Vero and Hep-2 cell lines are the most commonly used media for co-cultivation both in-house and commercial diagnostic kits production. However, HeLa cells which are easily supplied and grown, also can easily be infected by B.henselae. The aim of this study was to compare the performances of antigens obtained by co-cultivation of B.henselae ATCC 49882 (Houston-1) in Vero and HeLa Cells in IFA serology. Out of 381 sera samples, 127 (33.3%) were found positive and 195 (51.2%) were found negative by IFA performed by both cell line co-cultivations. The total agreement between the methods were found as 84.5% (322/381), and Cohen kappa value was calculated as 0.68 (strong, coherent). As a result, HeLa cells were found to be useful for the preparation of B.henselae antigens to be used in IFA for the serologic diagnosis of B.henselae infections. However different genotype strains and cross reactions with other infectious agents should be investigated by further studies before routine applications of HeLa cell co-cultivations procedure is established. | URI: | https://hdl.handle.net/11499/5964 | ISSN: | 0374-9096 |
Appears in Collections: | PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection Tıp Fakültesi Koleksiyonu TR Dizin İndeksli Yayınlar Koleksiyonu / TR Dizin Indexed Publications Collection WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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