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https://hdl.handle.net/11499/5968
Title: | Mannitol attenuates acute lung injury induced by infrarenal aortic occlusion-reperfusion in rats | Authors: | Teke, Z. Adali, F. Kelten, E.C. Enli, Yaşar Sackan, K.G. Karaman, Kerem Akbulut, M. |
Keywords: | Acute lung injury Aortic occlusion Glutathione Ischemia/reperfusion Malondialdehyde Myeloperoxidase Neutrophil Pulmonary microvascular dysfunction Superoxide dismutase biological marker Evans blue glutathione malonaldehyde mannitol myeloperoxidase superoxide dismutase acute lung injury animal experiment animal model animal tissue antioxidant activity arterial gas article controlled study cytodiagnosis drug effect drug efficacy dry weight enzyme activity histopathology kidney artery stenosis lung edema lung lavage lung parenchyma lung sequestration male nonhuman oxidative stress phlebotomy rat reperfusion injury Acute Kidney Injury Animals Aorta, Abdominal Biological Markers Diuretics, Osmotic Male Mannitol Neutrophils Oxidative Stress Peroxidase Pulmonary Edema Rats Rats, Wistar Reactive Oxygen Species Renal Artery Reperfusion Injury Superoxide Dismutase |
Abstract: | Purpose: Mannitol is used as a treatment for ischemia/reperfusion (IR) injury of various organs in humans, despite the fact that its effectiveness in vivo is still disputed. The purpose of this study was to determine the effects of mannitol on acute lung injury (ALI) induced by infrarenal aortic occlusion. Methods: Male Wistar-albino rats were allocated into five groups: (i) sham-operated group, which received a laparotomy without IR injury (n = 12); (ii) IR group, which received 3 h of ischemia followed by 2 h of reperfusion (n = 12); (iii) IR + inferior caval phlebotomy (ICP) group, which was identical to group 2 except for 1 ml of blood aspiration from the inferior caval vein (n = 12); (iv) IR + mannitol-treated group, for which rats were subjected to IR and received a bolus injection of mannitol (n = 12); and (v) IR + ICP + mannitol-treated group, which underwent the same procedures as described for the previous groups. Arterial blood gas parameters were studied and bronchoalveolar lavage (BAL) was performed. Evans blue dye was injected into half of the rats. We biochemically assessed the degree of pulmonary tissue injury by investigating oxidative stress markers and enzymatic and nonenzymatic antioxidant markers, and evaluated ALI by establishing pulmonary leukosequestration and ALI scoring, histopathologically. Pulmonary edema was estimated by using Evans blue dye extravasation and wet/dry weight ratios. Results: Hypertonic mannitol treatment significantly reduced oxidative stress markers, and significantly increased enzymatic and nonenzymatic antioxidant markers in the lung tissues (P < 0.05). Arterial blood gas parameters were significantly ameliorated (P < 0.05), the BAL cytology was significantly better (P < 0.05), pulmonary leukosequestration and ALI scores were significantly decreased (P < 0.05), and pulmonary edema was significantly alleviated (P < 0.05) by mannitol administration. Conclusion: This study clearly showed that mannitol treatment significantly attenuated the aortic IR-induced ALI. Further clinical studies are required to clarify whether mannitol has a useful role in ALI during surgeries in which IR-induced organ injury occurs. © 2011 Springer. | URI: | https://hdl.handle.net/11499/5968 https://doi.org/10.1007/s00595-010-4394-x |
ISSN: | 0941-1291 |
Appears in Collections: | PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection Tıp Fakültesi Koleksiyonu WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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