Microfluidic Channel Volume Optimization and Calibration in Accordance With Iso 17025 and Iso 4787 Standards

Abstract

Advances in three-dimensional printing (3DP) have enabled low-cost, assembly-free fabrication of microfluidic devices. However, internal microchannel precision remains a key challenge. This study presents a calibration method for SLA-based microfluidic devices fabricated via DLP 3DP using a standard transparent resin. ISO 17025 and ISO 4787:2021 were applied to assess dimensional and volumetric accuracy. Ten models with 500 mu m and 1000 mu m channels were tested. While 1000 mu m channels showed high accuracy and repeatability, 500 mu m channels were often blocked due to resin curing. The method offers a reproducible, standards-based framework for evaluating 3DP microfluidics using accessible materials.Graphical AbstractISO 17025 and ISO 4787 Compliant Microfluidic Volume Optimization Supporting UN SDG 9