Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/6241
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dc.contributor.authorArikan, S.Y.-
dc.contributor.authorAtalay, A.-
dc.contributor.authorAtalay, E.O.-
dc.date.accessioned2019-08-16T12:05:16Z-
dc.date.available2019-08-16T12:05:16Z-
dc.date.issued2010-
dc.identifier.issn1811-9727-
dc.identifier.urihttps://hdl.handle.net/11499/6241-
dc.identifier.urihttps://doi.org/10.3923/ijcr.2010.251.256-
dc.description.abstractThe ability of a peptide to target a specific protein in vitro has a potential for recognizing the cell membrane structure, protein-protein and receptor-ligand interaction. On the basis of molecular interactions, cell specific peptides were selected via phage library approach and their functions on the cells were determined by XTT based viability assay. We aimed to find phage displayed peptides from 12-mer peptide library that interact with K562-dox cell membrane in association with cellular functions and to study the effects of peptides selected from artificial peptide library on K562-dox cell viability. Peptides recognizing K562-dox cells were identified according to peptide sequences and amino acid properties. We selected 29 different phages from biopannings with K562-dox cells. Three clones were identified (KPB7,KPB10, KPP8) and their negative effects on cell viability were determined by XTT assay. According to our cell viability assay results, selected phages were effected negatively to the viability of the K562-dox cells. Depending on the present results, peptides were obtained that could be potential candidates for molecular recognition and cell targeting approaches. © 2010 Academic Journals Inc.en_US
dc.language.isoenen_US
dc.relation.ispartofInternational Journal of Cancer Researchen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCell viabilityen_US
dc.subjectK562-dox cellsen_US
dc.subjectPeptide librariesen_US
dc.subjectPhage displayen_US
dc.subjectXtt assayen_US
dc.subjectdoxorubicinen_US
dc.subjectpeptide libraryen_US
dc.subjectanimal cellen_US
dc.subjectarticleen_US
dc.subjectbacteriophageen_US
dc.subjectbiopanningen_US
dc.subjectcell cloneen_US
dc.subjectcell functionen_US
dc.subjectcell strain K 562en_US
dc.subjectcell structureen_US
dc.subjectcell viabilityen_US
dc.subjectcontrolled studyen_US
dc.subjectDNA isolationen_US
dc.subjectDNA sequenceen_US
dc.subjecthydrophobicityen_US
dc.subjectmolecular libraryen_US
dc.subjectnonhumanen_US
dc.subjectnucleotide sequenceen_US
dc.subjectphage displayen_US
dc.subjectphage KPB10en_US
dc.subjectphage KPB7en_US
dc.subjectphage KPP8en_US
dc.subjectphysical chemistryen_US
dc.subjectprotein interactionen_US
dc.titleInteraction of peptides selected from artificial peptide library with doxorubicin resistant K562 cellsen_US
dc.typeArticleen_US
dc.identifier.volume6en_US
dc.identifier.issue4en_US
dc.identifier.startpage251
dc.identifier.startpage251en_US
dc.identifier.endpage256en_US
dc.authorid0000-0001-7987-9078-
dc.authorid0000-0001-6272-9380-
dc.identifier.doi10.3923/ijcr.2010.251.256-
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.scopus2-s2.0-78149451082en_US
dc.identifier.scopusqualityQ3-
dc.ownerPamukkale University-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.grantfulltextnone-
item.languageiso639-1en-
item.openairetypeArticle-
item.fulltextNo Fulltext-
item.cerifentitytypePublications-
crisitem.author.dept14.03. Basic Medical Sciences-
Appears in Collections:Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Tıp Fakültesi Koleksiyonu
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