Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/6342
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dc.contributor.authorBuzzega, D.-
dc.contributor.authorPederzoli, F.-
dc.contributor.authorMacCari, F.-
dc.contributor.authorAslan, D.-
dc.contributor.authorTürk, Mehmet-
dc.contributor.authorVolpi, N.-
dc.date.accessioned2019-08-16T12:06:22Z
dc.date.available2019-08-16T12:06:22Z
dc.date.issued2010-
dc.identifier.issn1434-6621-
dc.identifier.urihttps://hdl.handle.net/11499/6342-
dc.identifier.urihttps://doi.org/10.1515/CCLM.2010.217-
dc.description.abstractBackground: Glycosaminoglycans (GAGs) are natural complex polysaccharides that are important in several pathological processes. Urinary GAGs have long been investigated for their possible modifications in many pathological conditions. In some cases, they have been found to have diagnostic utility. As a result, the measurement of GAGs in urine is gradually gaining importance. Cetylpyridinium chloride (CPC) and cetyltrimethylammonium bromide (CETAB) are generally used to extract urinary GAGs prior to analysis. In this study, we evaluated the extraction of human urinary GAGs using CPC in comparison with CETAB. Methods: Extracted urinary GAGs were qualitatively and quantitatively analyzed by agarose-gel electrophoresis in the presence of sequential staining and densitometric scanning. This procedure was able to give more reproducible and reliable results for urinary GAGs, and high performance liquid chromatography (HPLC) was used for the evaluation of chondroitin sulfate (CS) disaccharides. Results: Differences were observed between CPC and CETAB extract protocols. The absolute amount of CS evaluated by electrophoresis was found to be similar for the two protocols. However, the heparan sulfate (HS) concentration was calculated to be approximately 3.3 times greater for CPC than CETAB. When calculated in relative percentage, 33.6% HS was determined for CPC and 10.0% for CETAB. These results show a quantitative expression for greater recovery of HS by using CPC protocol than CETAB. No significant differences were found between CS quantified by agarose-gel and HPLC. In addition, no differences were observed for the CS disaccharide composition purified by using CPC or CETAB, and quite similar results were observed for 4s/6s disaccharide ratios and charge density values. Conclusions: Extract procedures for urinary GAGs using CPC or CETAB are able to recover similar amounts of CS quantified by agarose-gel electrophoresis and HPLC. However, CPC yields greater recovery of HS than the CETAB protocol; an increase of approximately 3.3 times as evaluated by electrophoresis. This different capacity of HS extraction between CPC and CETAB should be considered when urinary GAGs of subjects affected by various diseases and related pharmacological treatments are considered, or meta-analysis is performed comparing various studies and trials performed under different experimental conditions. © 2010 by Walter de Gruyter Berlin New York.en_US
dc.language.isoenen_US
dc.relation.ispartofClinical Chemistry and Laboratory Medicineen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectchondroitin sulfateen_US
dc.subjectglycosaminoglycansen_US
dc.subjectheparan sulfateen_US
dc.subjecturine analysisen_US
dc.subjectcetrimideen_US
dc.subjectcetylpyridinium salten_US
dc.subjectglycosaminoglycanen_US
dc.subjectadulten_US
dc.subjectagar gel electrophoresisen_US
dc.subjectageden_US
dc.subjectarticleen_US
dc.subjectcontrolled studyen_US
dc.subjectdensitometryen_US
dc.subjectdensityen_US
dc.subjectextractionen_US
dc.subjectfemaleen_US
dc.subjectglycosaminoglycan urine levelen_US
dc.subjecthigh performance liquid chromatographyen_US
dc.subjecthumanen_US
dc.subjecthuman experimenten_US
dc.subjectintermethod comparisonen_US
dc.subjectmaleen_US
dc.subjectpriority journalen_US
dc.subjectqualitative analysisen_US
dc.subjectquantitative analysisen_US
dc.subjectreliabilityen_US
dc.subjectstainingen_US
dc.subjecturine levelen_US
dc.subjectCetrimonium Compoundsen_US
dc.subjectCetylpyridiniumen_US
dc.subjectChondroitin Sulfatesen_US
dc.subjectChromatography, High Pressure Liquiden_US
dc.subjectElectrophoresis, Agar Gelen_US
dc.subjectGlycosaminoglycansen_US
dc.subjectHeparitin Sulfateen_US
dc.subjectHumansen_US
dc.titleComparison of cetylpyridinium chloride and cetyltrimethylammonium bromide extractive procedures for quantification and characterization of human urinary glycosaminoglycansen_US
dc.typeArticleen_US
dc.identifier.volume48en_US
dc.identifier.issue8en_US
dc.identifier.startpage1133
dc.identifier.startpage1133en_US
dc.identifier.endpage1139en_US
dc.identifier.doi10.1515/CCLM.2010.217-
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.pmid20441479en_US
dc.identifier.scopus2-s2.0-77955456865en_US
dc.identifier.wosWOS:000280584500014en_US
dc.identifier.scopusqualityQ2-
dc.ownerPamukkale University-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.grantfulltextnone-
item.languageiso639-1en-
item.openairetypeArticle-
item.fulltextNo Fulltext-
item.cerifentitytypePublications-
crisitem.author.dept14.03. Basic Medical Sciences-
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Tıp Fakültesi Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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