Please use this identifier to cite or link to this item:
https://hdl.handle.net/11499/6496
Title: | RNA interference-mediated URG4 gene silencing diminishes cyclin D1 mRNA expression in HepG2 cells. | Authors: | Satiroglu-Tufan, Naciye Lale Dodurga, Yavuz Gok, D. Cetinkaya, A. Feitelson, M.A. |
Keywords: | cyclin D1 messenger RNA tumor protein URG4 protein, human article cell proliferation cell strain HepG2 gene silencing genetics human physiology reverse transcription polymerase chain reaction RNA interference Cell Proliferation Cyclin D1 Gene Silencing Hep G2 Cells Humans Neoplasm Proteins Reverse Transcriptase Polymerase Chain Reaction RNA Interference RNA, Messenger |
Abstract: | Up-regulated gene 4 (URG4), stimulated by HBxAg, is a novel gene located on chromosome 7 (7p13). The full-length URG4 clone is 3.607 kb and encodes a polypeptide of 922 amino acids, with a molecular weight of 104 kDa (GeneID: 55665). It promotes cell growth, growth factor-independent survival, and anchorage-independent growth in HepG2 cells, and it accelerates tumor formation in nude mice. Hence, URG4 may be a natural effector of HBxAg and a putative oncogene that contributes to multi-step hepatocarcinogenesis. Cyclin D1 is frequently over-expressed in hepatocellular carcinoma, exhibiting a number of malignant phenotypes. We found that down-regulation of URG4 through RNA interference-mediated silencing suppressed cell proliferation in HepG2 cells. Over-expression of URG4 up-regulated cyclin D1 mRNA expression, whereas RNA interference-mediated URG4 silencing diminished cyclin D1 mRNA expression in HepG2 cells. The data suggest that URG4 may play an important role in the development of hepatocellular carcinoma by partially regulating the expression of cyclin D1 and has potential for use as a therapeutic target for hepatocellular carcinoma. | URI: | https://hdl.handle.net/11499/6496 https://doi.org/10.4238/vol9-3gmr872 |
ISSN: | 1676-5680 |
Appears in Collections: | PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection Tıp Fakültesi Koleksiyonu WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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