Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/6496
Title: RNA interference-mediated URG4 gene silencing diminishes cyclin D1 mRNA expression in HepG2 cells.
Authors: Satiroglu-Tufan, Naciye Lale
Dodurga, Yavuz
Gok, D.
Cetinkaya, A.
Feitelson, M.A.
Keywords: cyclin D1
messenger RNA
tumor protein
URG4 protein, human
article
cell proliferation
cell strain HepG2
gene silencing
genetics
human
physiology
reverse transcription polymerase chain reaction
RNA interference
Cell Proliferation
Cyclin D1
Gene Silencing
Hep G2 Cells
Humans
Neoplasm Proteins
Reverse Transcriptase Polymerase Chain Reaction
RNA Interference
RNA, Messenger
Abstract: Up-regulated gene 4 (URG4), stimulated by HBxAg, is a novel gene located on chromosome 7 (7p13). The full-length URG4 clone is 3.607 kb and encodes a polypeptide of 922 amino acids, with a molecular weight of 104 kDa (GeneID: 55665). It promotes cell growth, growth factor-independent survival, and anchorage-independent growth in HepG2 cells, and it accelerates tumor formation in nude mice. Hence, URG4 may be a natural effector of HBxAg and a putative oncogene that contributes to multi-step hepatocarcinogenesis. Cyclin D1 is frequently over-expressed in hepatocellular carcinoma, exhibiting a number of malignant phenotypes. We found that down-regulation of URG4 through RNA interference-mediated silencing suppressed cell proliferation in HepG2 cells. Over-expression of URG4 up-regulated cyclin D1 mRNA expression, whereas RNA interference-mediated URG4 silencing diminished cyclin D1 mRNA expression in HepG2 cells. The data suggest that URG4 may play an important role in the development of hepatocellular carcinoma by partially regulating the expression of cyclin D1 and has potential for use as a therapeutic target for hepatocellular carcinoma.
URI: https://hdl.handle.net/11499/6496
https://doi.org/10.4238/vol9-3gmr872
ISSN: 1676-5680
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Tıp Fakültesi Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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