Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/6863
Title: Comparison of the Therapeutic Efficacy of Linezolid and Vancomycin and Correlation of Serum and Tissue Malondialdehyde and Myeloperoxidase in an Experimental Mediastinitis Model
Authors: Sacar, S.
Sacar, M.
Aybek, Hülya
Turgut, Hüseyin
Önem, Gökhan
Cevahir, Nural
Teke, Z.
Keywords: experimental
linezolid
mediastinitis
vancomycin
cefazolin
malonaldehyde
myeloperoxidase
animal experiment
animal model
animal tissue
antibiotic prophylaxis
antibiotic therapy
article
bacterial count
colony forming unit
comparative study
control group
controlled study
correlation analysis
disease model
drug effect
drug efficacy
heart
histochemistry
immune system
infection prevention
inoculation
kidney
liver
lung
male
mediastinum
methicillin resistant Staphylococcus aureus
nonhuman
pleura cavity
priority journal
protein blood level
randomization
rat
sternotomy
tissue level
Wistar rat
Acetamides
Animals
Anti-Bacterial Agents
Disease Models, Animal
Male
Malondialdehyde
Mediastinitis
Methicillin-Resistant Staphylococcus aureus
Oxazolidinones
Peroxidase
Rats
Rats, Wistar
Vancomycin
Abstract: Background: We aimed to investigate the therapeutic efficacy of linezolid in an experimental mediastinitis model and to compare it with vancomycin, which is commonly used. The objective of this study was also to evaluate the role of the immune system in mediastinitis. Materials and methods: Fifty adult Wistar rats were randomly divided into five groups: an uncontaminated and contaminated untreated control groups; a group that received sefazolin prophylaxis; and two groups treated with vancomycin or linezolid. Median sternotomy without access to pleural spaces was performed on all rats. All groups, except the uncontaminated one, were inoculated with 0.5 mL 108 colony-forming units/mL methicillin-resistant Staphylococcus aureus in the mediastinal and sternal layers. Postoperatively, vancomycin and linezolid groups were given antibiotic treatment for 7 d, starting 24 h after the end of the procedure. After 7-d treatment tissue samples from the upper ends of the sternotomy line and mediastinum were obtained and evaluated microbiologically. Additionally, serum, heart, lung, liver, kidney, and mediastinal tissues samples were obtained to determine malondialdehyde (MDA) and myeloperoxidase (MPO). Results: The study showed that either vancomycin or linezolid successfully reduced bacterial counts in mediastinum and sternotomy line. MDA and MPO levels were found to be decreased in the treated groups. There was a positive correlation between serum and tissues MDA and MPO in all of the groups. Conclusions: Our study showed that linezolid appears to be a promising option for treating mediastinitis due to methicillin-resistant S. aureus. Additionally, it was demonstrated that a wide inflammatory process occurred after mediastinitis. © 2009 Elsevier Inc. All rights reserved.
URI: https://hdl.handle.net/11499/6863
https://doi.org/10.1016/j.jss.2008.03.040
ISSN: 0022-4804
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Tıp Fakültesi Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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