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https://hdl.handle.net/11499/6863
Title: | Comparison of the Therapeutic Efficacy of Linezolid and Vancomycin and Correlation of Serum and Tissue Malondialdehyde and Myeloperoxidase in an Experimental Mediastinitis Model | Authors: | Sacar, S. Sacar, M. Aybek, Hülya Turgut, Hüseyin Önem, Gökhan Cevahir, Nural Teke, Z. |
Keywords: | experimental linezolid mediastinitis vancomycin cefazolin malonaldehyde myeloperoxidase animal experiment animal model animal tissue antibiotic prophylaxis antibiotic therapy article bacterial count colony forming unit comparative study control group controlled study correlation analysis disease model drug effect drug efficacy heart histochemistry immune system infection prevention inoculation kidney liver lung male mediastinum methicillin resistant Staphylococcus aureus nonhuman pleura cavity priority journal protein blood level randomization rat sternotomy tissue level Wistar rat Acetamides Animals Anti-Bacterial Agents Disease Models, Animal Male Malondialdehyde Mediastinitis Methicillin-Resistant Staphylococcus aureus Oxazolidinones Peroxidase Rats Rats, Wistar Vancomycin |
Abstract: | Background: We aimed to investigate the therapeutic efficacy of linezolid in an experimental mediastinitis model and to compare it with vancomycin, which is commonly used. The objective of this study was also to evaluate the role of the immune system in mediastinitis. Materials and methods: Fifty adult Wistar rats were randomly divided into five groups: an uncontaminated and contaminated untreated control groups; a group that received sefazolin prophylaxis; and two groups treated with vancomycin or linezolid. Median sternotomy without access to pleural spaces was performed on all rats. All groups, except the uncontaminated one, were inoculated with 0.5 mL 108 colony-forming units/mL methicillin-resistant Staphylococcus aureus in the mediastinal and sternal layers. Postoperatively, vancomycin and linezolid groups were given antibiotic treatment for 7 d, starting 24 h after the end of the procedure. After 7-d treatment tissue samples from the upper ends of the sternotomy line and mediastinum were obtained and evaluated microbiologically. Additionally, serum, heart, lung, liver, kidney, and mediastinal tissues samples were obtained to determine malondialdehyde (MDA) and myeloperoxidase (MPO). Results: The study showed that either vancomycin or linezolid successfully reduced bacterial counts in mediastinum and sternotomy line. MDA and MPO levels were found to be decreased in the treated groups. There was a positive correlation between serum and tissues MDA and MPO in all of the groups. Conclusions: Our study showed that linezolid appears to be a promising option for treating mediastinitis due to methicillin-resistant S. aureus. Additionally, it was demonstrated that a wide inflammatory process occurred after mediastinitis. © 2009 Elsevier Inc. All rights reserved. | URI: | https://hdl.handle.net/11499/6863 https://doi.org/10.1016/j.jss.2008.03.040 |
ISSN: | 0022-4804 |
Appears in Collections: | PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection Tıp Fakültesi Koleksiyonu WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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