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https://hdl.handle.net/11499/7039
Title: | Effect of thrombocytapheresis on blood rheology in healthy donors: Role of nitric oxide | Authors: | Bor-Küçükatay, Melek Keskin, A. Akdam, Hakan Kabukcu-Hacioglu, S. Erken, Gülten Atsak, P. Küçükatay, Vural |
Keywords: | Erythrocyte Hemorheology Nitric oxide Thrombocytapheresis albumin cholesterol fibrinogen glucose hemoglobin nitric oxide nitroprusside sodium protein triacylglycerol albumin blood level apheresis article biochemistry blood rheology blood sampling blood viscosity cell protection cholesterol blood level erythrocyte erythrocyte aggregation fibrinogen blood level glucose blood level hematocrit hematology hemoglobin blood level human male microvasculature normal human protein blood level thrombocyte preservation thrombocytopheresis triacylglycerol blood level viscometer Adult Anticoagulants Blood Donors Blood Glucose Blood Proteins Blood Viscosity Cholesterol Erythrocyte Aggregation Erythrocyte Deformability Fibrinogen Hematocrit Humans Male Microcirculation Nitric Oxide Nitric Oxide Donors Nitroprusside Plateletpheresis Serum Albumin Triglycerides |
Abstract: | Platelet transfusions are increasingly being used to treat thrombocytopenic conditions. Because of anticoagulation, changes in blood composition and extracorporeal circulation, donor apheresis may cause alterations in hemorheology. This study aimed at investigating the effects of thrombocytapheresis on donor blood rheology. The effect of nitric oxide (NO) on donor red blood cell (RBC) deformability after thrombocytapheresis was also studied. Platelets were collected by a Haemonetics MCS 3p cell seperator. Blood samples were obtained before and 15 min after thrombocytapheresis. RBC deformability and aggregation were measured using an ektacytometer, whole blood viscosity (WBV) was determined with a cone-plate rotational viscometer. Donor RBCs were shown to be less deformable at all stress levels except 0.30 Pa after thrombocytapheresis and NO donor sodium nitroprusside (SNP, 10-6 M) reversed the reduced deformability caused by thrombocytapheresis. It was observed that donor apheresis induces a decrement in RBC aggregation and WBV measured at standard hematocrit (Hct). No significant alterations were observed in WBV values determined at native Hct values. Thrombocytapheresis also resulted in a decrement in fibrinogen, total protein, cholesterol and albumin levels whereas Hct was found to be increased and serum glucose, triglyceride, hemoglobin levels unaltered after apheresis. These results suggest that, thrombocytapheresis causes alterations in hemorheological parameters and hence in the perfusion of the microvasculature of the donors and NO appears to have a protective effect on the impairment observed in RBC deformability. Crown Copyright © 2008. | URI: | https://hdl.handle.net/11499/7039 https://doi.org/10.1016/j.transci.2008.07.004 |
ISSN: | 1473-0502 |
Appears in Collections: | PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection Tıp Fakültesi Koleksiyonu WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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