Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/7039
Title: Effect of thrombocytapheresis on blood rheology in healthy donors: Role of nitric oxide
Authors: Bor-Küçükatay, Melek
Keskin, A.
Akdam, Hakan
Kabukcu-Hacioglu, S.
Erken, Gülten
Atsak, P.
Küçükatay, Vural
Keywords: Erythrocyte
Hemorheology
Nitric oxide
Thrombocytapheresis
albumin
cholesterol
fibrinogen
glucose
hemoglobin
nitric oxide
nitroprusside sodium
protein
triacylglycerol
albumin blood level
apheresis
article
biochemistry
blood rheology
blood sampling
blood viscosity
cell protection
cholesterol blood level
erythrocyte
erythrocyte aggregation
fibrinogen blood level
glucose blood level
hematocrit
hematology
hemoglobin blood level
human
male
microvasculature
normal human
protein blood level
thrombocyte preservation
thrombocytopheresis
triacylglycerol blood level
viscometer
Adult
Anticoagulants
Blood Donors
Blood Glucose
Blood Proteins
Blood Viscosity
Cholesterol
Erythrocyte Aggregation
Erythrocyte Deformability
Fibrinogen
Hematocrit
Humans
Male
Microcirculation
Nitric Oxide
Nitric Oxide Donors
Nitroprusside
Plateletpheresis
Serum Albumin
Triglycerides
Abstract: Platelet transfusions are increasingly being used to treat thrombocytopenic conditions. Because of anticoagulation, changes in blood composition and extracorporeal circulation, donor apheresis may cause alterations in hemorheology. This study aimed at investigating the effects of thrombocytapheresis on donor blood rheology. The effect of nitric oxide (NO) on donor red blood cell (RBC) deformability after thrombocytapheresis was also studied. Platelets were collected by a Haemonetics MCS 3p cell seperator. Blood samples were obtained before and 15 min after thrombocytapheresis. RBC deformability and aggregation were measured using an ektacytometer, whole blood viscosity (WBV) was determined with a cone-plate rotational viscometer. Donor RBCs were shown to be less deformable at all stress levels except 0.30 Pa after thrombocytapheresis and NO donor sodium nitroprusside (SNP, 10-6 M) reversed the reduced deformability caused by thrombocytapheresis. It was observed that donor apheresis induces a decrement in RBC aggregation and WBV measured at standard hematocrit (Hct). No significant alterations were observed in WBV values determined at native Hct values. Thrombocytapheresis also resulted in a decrement in fibrinogen, total protein, cholesterol and albumin levels whereas Hct was found to be increased and serum glucose, triglyceride, hemoglobin levels unaltered after apheresis. These results suggest that, thrombocytapheresis causes alterations in hemorheological parameters and hence in the perfusion of the microvasculature of the donors and NO appears to have a protective effect on the impairment observed in RBC deformability. Crown Copyright © 2008.
URI: https://hdl.handle.net/11499/7039
https://doi.org/10.1016/j.transci.2008.07.004
ISSN: 1473-0502
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Tıp Fakültesi Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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