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https://hdl.handle.net/11499/7041
Title: | Effects of Tempol, a Membrane-Permeable Radical Scavenger, on Local and Remote Organ Injuries Caused by Intestinal Ischemia/Reperfusion in Rats | Authors: | Teke, Z. Kabay, Burhan Özden, Akın Yenisey, C. Bir, Ferda Demirkan, N.C. Bicakci, T. |
Keywords: | acute lung injury glutathione intestinal mucosal injury ischemia/reperfusion malondialdehyde mesenteric ischemia myeloperoxidase neutrophil nitrate nitrite pulmonary microvascular dysfunction reperfusion injury Tempol Evans blue malonaldehyde scavenger tempol animal experiment animal model animal tissue article controlled study drug mechanism histopathology intestine injury intestine ischemia intestine mucosa laparotomy lung injury lung parenchyma male membrane permeability neutrophil chemotaxis nonhuman priority journal rat superior mesenteric artery obstruction Animals Capillary Permeability Cyclic N-Oxides Edema Free Radical Scavengers Glutathione Intestinal Mucosa Lung Male Malondialdehyde Mesenteric Artery, Superior Mesenteric Vascular Occlusion Neutrophils Nitrates Nitrites Peroxidase Rats Rats, Wistar Reperfusion Injury Respiratory Distress Syndrome, Adult Spin Labels |
Abstract: | Background: Tempol is a stable piperidine nitroxide of low molecular weight that permeates biological membranes and scavenges superoxide anions in vitro. In a variety of animal models, deleterious effects of reperfusion injury on both local and remote organs have been demonstrated. In this study, we aimed to investigate the effects of a membrane-permeable radical scavenger, Tempol, on local and remote organ injuries caused by intestinal ischemia/reperfusion (I/R) in rats. Materials and methods: Male Wistar-albino rats were randomized into three groups: (I) Sham-operated control group, laparotomy without I/R injury (n = 12); (II) Intestinal I/R group, 60 min of ischemia by superior mesenteric artery occlusion followed by 2-h of reperfusion (n = 12); and (III) I/R + Tempol-treated group, identical to I/R group except for Tempol administration, 30 mg/kg bolus injection 5 min before reperfusion, followed by an infusion of 30 mg/kg/h intravenously (n = 12). Histopathologically, intestinal mucosal lesions were assessed by Chiu's classification, and pulmonary parenchymal damage was appraised by pulmonary neutrophil infiltration and acute lung injury scaling. Biochemically, myeloperoxidase activity, malondialdehyde, glutathione, and nitrite/nitrate (NOx) levels were determined in both intestinal mucosa and lung parenchyma. Evans blue dye concentration and organ wet/dry weight ratios were used as a marker of organ edema. Animal survival was observed up to 1 week. Results: Intestinal mucosal lesions and pulmonary parenchymal damage were significantly attenuated with Tempol treatment, histopathologically (P < 0.05). Tempol administration significantly reduced myeloperoxidase activity and malondialdehyde levels, and also significantly increased glutathione and NOx levels of both intestinal and lung tissues, biochemically (P < 0.05). Evans blue dye extravasation and wet/dry weight ratios of organs were significantly reduced with Tempol injection (P < 0.05). The survival rates of rats in Tempol-treated group were significantly higher than that of I/R-treated group (P < 0.05). Conclusions: The present study suggests that Tempol administration significantly reduces both local and remote organ injuries caused by intestinal I/R before and throughout the reperfusion period. Further clinical studies are needed to clarify whether Tempol may be a useful therapeutic agent to use in particular operations where the reperfusion injury occurs. © 2008 Elsevier Inc. All rights reserved. | URI: | https://hdl.handle.net/11499/7041 https://doi.org/10.1016/j.jss.2007.12.791 |
ISSN: | 0022-4804 |
Appears in Collections: | PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection Tıp Fakültesi Koleksiyonu WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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