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https://hdl.handle.net/11499/7042
Title: | Activated Protein C Attenuates Intestinal Mucosal Injury After Mesenteric Ischemia/Reperfusion | Authors: | Teke, Z. Sacar, M. Yenisey, C. Atalay, A.O. Kavak, T. Erdem, Ergün |
Keywords: | activated protein C catalase glutathione glutathione peroxidase glutathione reductase ischemia/reperfusion malondialdehyde mesenteric ischemia mucosal injury myeloperoxidase nitrate/nitrite reperfusion injury xanthine oxidase D dimer drotrecogin interleukin 6 malonaldehyde nitrate nitrite oxidoreductase tumor necrosis factor alpha animal experiment animal model animal tissue article controlled study histopathology intestine injury intestine mucosa ischemia laparotomy male nonhuman priority journal rat reperfusion superior mesenteric artery obstruction survival Animals Anticoagulants Edema Fibrin Fibrinogen Degradation Products Glutathione Glutathione Reductase Interleukin-6 Intestinal Mucosa Male Malondialdehyde Mesenteric Artery, Superior Mesenteric Vascular Occlusion Nitrates Nitrites Peroxidases Protein C Rats Rats, Wistar Reperfusion Injury Tumor Necrosis Factor-alpha Warm Ischemia Xanthine Oxidase |
Abstract: | Background: Activated protein C (APC) is a serine protease with anticoagulant and ant-inflammatory activities. APC has been shown to attenuate deleterious effects of ischemia/reperfusion (I/R) injury in many organs. In this study, we aimed to investigate the effects of APC on intestinal mucosal injury induced by superior mesenteric occlusion. Materials and Methods: Male Wistar-albino rats were allocated into four groups: (1) sham-operated group, laparotomy without I/R injury (n = 12); (2) sham + APC group, identical to Group 1 except for APC treatment (n = 12); (3) I/R group, 60 min of ischemia followed by 3-h of reperfusion (n = 12); and (4) I/R + APC-treated group, 100 µg/kg injection of APC intravenously, 15 min before reperfusion (n = 12). We evaluated the degree of intestinal mucosal injury on a grading scale from 0 to 5, histopathologically, and by measuring activities of oxidative and antioxidative enzymes as well as nitrate/nitrite levels, biochemically. Intestinal edema was estimated by using wet/dry weight ratios. The plasma levels of proinflammatory cytokines and D-dimer were measured. Animal survival was observed up to 1 wk. Results: Intestinal mucosal injury scores were significantly decreased with APC administration (P < 0.05). APC treatment significantly reduced activities of oxidative enzymes and nitrate/nitrite levels in the intestinal tissues, and plasma levels of proinflammatory cytokines and D-dimer, and also significantly increased activities of antioxidative enzymes in the intestinal tissues (P < 0.05). Intestinal edema was significantly alleviated with APC treatment (P < 0.05). The survival rate of rats in the APC-treated group were significantly higher than that of the I/R-treated group (P < 0.05). Conclusions: This study clearly showed that APC treatment significantly attenuated intestinal mucosal injury caused by superior mesenteric ischemia/reperfusion. Further clinical studies are required to clarify whether APC has a useful role in reperfusion injury during particular surgeries in which I/R-induced organ injury occurs. © 2008 Elsevier Inc. All rights reserved. | URI: | https://hdl.handle.net/11499/7042 https://doi.org/10.1016/j.jss.2007.10.011 |
ISSN: | 0022-4804 |
Appears in Collections: | PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection Tıp Fakültesi Koleksiyonu WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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