Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/7042
Title: Activated Protein C Attenuates Intestinal Mucosal Injury After Mesenteric Ischemia/Reperfusion
Authors: Teke, Z.
Sacar, M.
Yenisey, C.
Atalay, A.O.
Kavak, T.
Erdem, Ergün
Keywords: activated protein C
catalase
glutathione
glutathione peroxidase
glutathione reductase
ischemia/reperfusion
malondialdehyde
mesenteric ischemia
mucosal injury
myeloperoxidase
nitrate/nitrite
reperfusion injury
xanthine oxidase
D dimer
drotrecogin
interleukin 6
malonaldehyde
nitrate
nitrite
oxidoreductase
tumor necrosis factor alpha
animal experiment
animal model
animal tissue
article
controlled study
histopathology
intestine injury
intestine mucosa
ischemia
laparotomy
male
nonhuman
priority journal
rat
reperfusion
superior mesenteric artery obstruction
survival
Animals
Anticoagulants
Edema
Fibrin Fibrinogen Degradation Products
Glutathione
Glutathione Reductase
Interleukin-6
Intestinal Mucosa
Male
Malondialdehyde
Mesenteric Artery, Superior
Mesenteric Vascular Occlusion
Nitrates
Nitrites
Peroxidases
Protein C
Rats
Rats, Wistar
Reperfusion Injury
Tumor Necrosis Factor-alpha
Warm Ischemia
Xanthine Oxidase
Abstract: Background: Activated protein C (APC) is a serine protease with anticoagulant and ant-inflammatory activities. APC has been shown to attenuate deleterious effects of ischemia/reperfusion (I/R) injury in many organs. In this study, we aimed to investigate the effects of APC on intestinal mucosal injury induced by superior mesenteric occlusion. Materials and Methods: Male Wistar-albino rats were allocated into four groups: (1) sham-operated group, laparotomy without I/R injury (n = 12); (2) sham + APC group, identical to Group 1 except for APC treatment (n = 12); (3) I/R group, 60 min of ischemia followed by 3-h of reperfusion (n = 12); and (4) I/R + APC-treated group, 100 µg/kg injection of APC intravenously, 15 min before reperfusion (n = 12). We evaluated the degree of intestinal mucosal injury on a grading scale from 0 to 5, histopathologically, and by measuring activities of oxidative and antioxidative enzymes as well as nitrate/nitrite levels, biochemically. Intestinal edema was estimated by using wet/dry weight ratios. The plasma levels of proinflammatory cytokines and D-dimer were measured. Animal survival was observed up to 1 wk. Results: Intestinal mucosal injury scores were significantly decreased with APC administration (P < 0.05). APC treatment significantly reduced activities of oxidative enzymes and nitrate/nitrite levels in the intestinal tissues, and plasma levels of proinflammatory cytokines and D-dimer, and also significantly increased activities of antioxidative enzymes in the intestinal tissues (P < 0.05). Intestinal edema was significantly alleviated with APC treatment (P < 0.05). The survival rate of rats in the APC-treated group were significantly higher than that of the I/R-treated group (P < 0.05). Conclusions: This study clearly showed that APC treatment significantly attenuated intestinal mucosal injury caused by superior mesenteric ischemia/reperfusion. Further clinical studies are required to clarify whether APC has a useful role in reperfusion injury during particular surgeries in which I/R-induced organ injury occurs. © 2008 Elsevier Inc. All rights reserved.
URI: https://hdl.handle.net/11499/7042
https://doi.org/10.1016/j.jss.2007.10.011
ISSN: 0022-4804
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Tıp Fakültesi Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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