Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/7418
Title: Potential utility of p63 expression in differential diagnosis of non-small-cell lung carcinoma and its effect on prognosis of the disease
Authors: Bir, Ferda
Altinboga, A.A.
Satiroglu-Tufan, Naciye Lale
Kaya, S.
Başer, Sevin
Yaren, Arzu
Keywords: Carcinoma
Diagnosis
Differential
Non-small-cell lung-pathology
Reverse transcriptase polymerase chain reaction-methods
protein p63
CKAP4 protein, human
membrane protein
messenger RNA
tumor marker
adenocarcinoma
adult
aged
article
Bowen disease
cancer prognosis
cancer staging
disease free survival
female
follow up
human
human tissue
immunohistochemistry
large cell carcinoma
lung non small cell cancer
major clinical study
male
metastasis
protein expression
reverse transcription polymerase chain reaction
squamous cell carcinoma
survival rate
tumor volume
Carcinoma, Non-Small-Cell Lung
differential diagnosis
metabolism
middle aged
procedures
prognosis
Turkey
Adenocarcinoma
Aged
Carcinoma, Large Cell
Carcinoma, Squamous Cell
Diagnosis, Differential
Female
Humans
Immunohistochemistry
Male
Membrane Proteins
Middle Aged
Prognosis
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger
Tumor Markers, Biological
Abstract: Background: P63 is a gene located in chromosome 3q27-29, which has been implicated in regulation of stem cell commitment and promotion of squamous differentiation in various tissues. The aim of this study was to investigate whether there was a correlation between p63 expression, differential diagnosis of lung carcinoma, and prognosis. Material/Methods: Immunohistochemical expression of p63 in 62 lung carcinomas was investigated and mRNA analysis using RT-PCR method was done in 6 selected cases. Results: When cases were evaluated for p63 staining, 24 of 25 (96%) squamous cell carcinomas were strongly positive. Six of 20 adenocarcinomas (25%) and 1 (100%) large cell carcinoma (except neuroendocrine carcinoma) were mildly positive. p63 staining was statistically significant in favor of squamous cell carcinoma than other tumors (p<0.001). Forty percent of squamous cell carcinomas had squamous carcinoma in situ, whereas adenocarcinomas had none. There was a significant statistical difference between squamous cell carcinoma and adenocarcinoma (p=0.002). p63 was strongly positive in all of 12 squamous carcinoma in situ cases. In 6 cases where mRNA analysis was performed by RT-PCR method, DNp63 was strongly positive in 3 squamous cell carcinomas, mildly positive in 1 adenocarcinoma, and negative in 1 carcinoid tumor. TAp63 was strongly positive in non-tumoral lung tissue but negative in all tumors, except 1 squamous cell carcinoma. Conclusions: Our data suggest that poorly differentiated squamous cell carcinoma had strong and widespread staining for immunohistochemical expression of p63. Therefore, p63 can be a useful marker in differentiating squamous cell carcinoma from poorly differentiated adenocarcinoma and squamous cell carcinoma from large cell neuroendocrine carcinoma. © Med Sci Monit.
URI: https://hdl.handle.net/11499/7418
https://doi.org/10.12659/MSM.890394
ISSN: 1234-1010
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Tıp Fakültesi Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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