Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/7443
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dc.contributor.authorDodurga, Yavuz-
dc.contributor.authorGündoğdu, Gülşah-
dc.contributor.authorTekin, V.-
dc.contributor.authorKoc, T.-
dc.contributor.authorSatiroglu-Tufan, Naciye Lale-
dc.contributor.authorBağcı, Gülseren-
dc.contributor.authorKüçükatay, Vural-
dc.date.accessioned2019-08-16T12:29:36Z
dc.date.available2019-08-16T12:29:36Z
dc.date.issued2014-
dc.identifier.issn0301-4851-
dc.identifier.urihttps://hdl.handle.net/11499/7443-
dc.identifier.urihttps://doi.org/10.1007/s11033-014-3330-3-
dc.description.abstractValproic acid (VPA), used for the treatment of epilepsy and bipolar disorder, regulates several signaling pathways in brain cells. The up-regulated gene 4 (URG4/URGCP) is a novel gene located on 7p13. URG4/URGCP stimulates cyclin D1 (CCND1) mRNA expression, and URG4/URGCP silencing diminishes CCND1 mRNA expression in HepG2 cells. This study was performed to investigate the anti-cancer mechanism of action of VPA by analyzing the expression of novel gene URG4/URGCP, CCND1, p21, p53, p65 (RelA), Bax, and Bcl-2 in SHSY5Y neuroblastoma (NB) cancer cells. Cytotoxic effects of VPA in SHSY5Y were noticed in time and dose dependent manner with the IC50 doses within the range of 0.5-10 mM. IC50 doses in the SHSY5Y were detected as 7.5 mM. Expression profiles were determined by semi quantitative RT-PCR and URG4/URGCP protein change by western blot analysis. Our results suggest that VPA induces cell cycle arrest in SHSY5Y due to the decrease in URG4/URGCP, CCND1 gene expression and the increase in p65. To conclude, VPA may be a prospective agent for the treatment of NB as a single agent or in combination with other drugs. Thus, more studies should be designed to find a safe dose with the best effects of VPA. © 2014 Springer Science+Business Media.en_US
dc.language.isoenen_US
dc.publisherKluwer Academic Publishersen_US
dc.relation.ispartofMolecular Biology Reportsen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectNeuroblastomaen_US
dc.subjectURG4/URGCPen_US
dc.subjectValproic aciden_US
dc.subjectcyclin D1en_US
dc.subjectprotein Baxen_US
dc.subjectprotein bcl 2en_US
dc.subjectprotein p21en_US
dc.subjectprotein p53en_US
dc.subjectsynaptotagmin Ien_US
dc.subjecttranscription factor RelAen_US
dc.subjectvalproic aciden_US
dc.subjectantineoplastic agenten_US
dc.subjectapoptosis regulatory proteinen_US
dc.subjectCCND1 protein, humanen_US
dc.subjectRELA protein, humanen_US
dc.subjecttumor proteinen_US
dc.subjectURG4 protein, humanen_US
dc.subjectantineoplastic activityen_US
dc.subjectarticleen_US
dc.subjectBax geneen_US
dc.subjectCCND1 geneen_US
dc.subjectcell cycle arresten_US
dc.subjectcell deathen_US
dc.subjectcell proliferationen_US
dc.subjectconcentration responseen_US
dc.subjectcontrolled studyen_US
dc.subjectdown regulationen_US
dc.subjectdrug cytotoxicityen_US
dc.subjectgeneen_US
dc.subjectgene expressionen_US
dc.subjectgene expression profilingen_US
dc.subjectgene silencingen_US
dc.subjectgenetic analysisen_US
dc.subjecthumanen_US
dc.subjecthuman cellen_US
dc.subjectIC 50en_US
dc.subjectneuroblastoma cell lineen_US
dc.subjectp21 geneen_US
dc.subjectp65 geneen_US
dc.subjectprotein expressionen_US
dc.subjectproto oncogeneen_US
dc.subjectreal time polymerase chain reactionen_US
dc.subjecttumor suppressor geneen_US
dc.subjectURG4 geneen_US
dc.subjectagonistsen_US
dc.subjectantagonists and inhibitorsen_US
dc.subjectcell survivalen_US
dc.subjectdrug effectsen_US
dc.subjectgene expression regulationen_US
dc.subjectgeneticsen_US
dc.subjectmetabolismen_US
dc.subjectnerve cellen_US
dc.subjectpathologyen_US
dc.subjectsignal transductionen_US
dc.subjecttumor cell lineen_US
dc.subjectAntineoplastic Agentsen_US
dc.subjectApoptosis Regulatory Proteinsen_US
dc.subjectCell Line, Tumoren_US
dc.subjectCell Proliferationen_US
dc.subjectCell Survivalen_US
dc.subjectCyclin D1en_US
dc.subjectGene Expression Regulation, Neoplasticen_US
dc.subjectHumansen_US
dc.subjectNeoplasm Proteinsen_US
dc.subjectNeuronsen_US
dc.subjectSignal Transductionen_US
dc.subjectTranscription Factor RelAen_US
dc.subjectValproic Aciden_US
dc.titleValproic acid inhibits the proliferation of SHSY5Y neuroblastoma cancer cells by downregulating URG4/URGCP and CCND1 gene expressionen_US
dc.typeArticleen_US
dc.identifier.volume41en_US
dc.identifier.issue7en_US
dc.identifier.startpage4595
dc.identifier.startpage4595en_US
dc.identifier.endpage4599en_US
dc.authorid0000-0002-4936-5954-
dc.authorid0000-0002-9924-5176-
dc.authorid0000-0001-9399-0960-
dc.authorid0000-0002-6850-6281-
dc.identifier.doi10.1007/s11033-014-3330-3-
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.pmid24652203en_US
dc.identifier.scopus2-s2.0-84903391401en_US
dc.identifier.wosWOS:000338283000044en_US
dc.identifier.scopusqualityQ2-
dc.ownerPamukkale University-
item.languageiso639-1en-
item.openairetypeArticle-
item.grantfulltextnone-
item.cerifentitytypePublications-
item.fulltextNo Fulltext-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
crisitem.author.dept14.03. Basic Medical Sciences-
crisitem.author.dept14.03. Basic Medical Sciences-
crisitem.author.dept14.02. Internal Medicine-
crisitem.author.dept14.03. Basic Medical Sciences-
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Tıp Fakültesi Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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