Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/7444
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dc.contributor.authorŞengül, Mustafa-
dc.contributor.authorErgin, Çağrı-
dc.contributor.authorKartal, T.-
dc.date.accessioned2019-08-16T12:29:36Z
dc.date.available2019-08-16T12:29:36Z
dc.date.issued2014-
dc.identifier.issn0374-9096-
dc.identifier.urihttps://hdl.handle.net/11499/7444-
dc.identifier.urihttps://doi.org/10.5578/mb.7450-
dc.description.abstractCryptococcus neofomans is an encapsulated yeast-like fungus that causes life-threatening infections, especially in immunosuppresive patients. C.neoformans infection is believed to be acquired via inhalation of aerosolized particles from the environment. Avian guano, decaying tree hollows and soil are the related known environmental niches. Brown pigmented yeast growth from the precursors in growth media is an important step for the identification and isolation of C.neoformans. Seeds of plants in nature are preferred owing to easy accessibility and low costs for the preperation of such media. Guizotia abysinicca (Niger seed) as Staib agar, Helianthus annus (Sunflower) as Pal's medium, Brassica nigra (Mustard) agar, tobacco agar, Mucuna pruriens (Velvet bean) seed agar, Perilla frutescens (Beefsteak plant) seed agar, Rubus fruticosus (Blackberry) agar and ground red hot pepper agar are pigment-based selective media for the differentiation of C.neoformans. The aim of this study was to observe the pigment production of Cneoformans in a new medium based on eggplant (Solanum melongena) and also to compare its performance with the simplified Staib, Pal's and tobacco agar for isolation from the environment. Three different eggplant-based medium (S.melongena Melanzaza viserba, S.melongena Pinstripe F1 and S.ovigerum Ivory F1) were included in the study. Pigment-forming eggplant medium, simplified Staib agar, Pal's agar and tobacco agar were used for the cultivation of the environmental swabbed samples from 19 Eucalyptus camaldulensis trunk hollows in continuous colonization region. While pigment formation were observed with S.melongena Melanzaza viserba and S.melongena Pinstripe F1 containing media, S.ovigerum Ivory F1 medium was found to be non-reactive. In colonization area (Gökova-Akyaka, Turkey), 11 (57.9%) out of 19 E.camaldulensis samples were positive with simplified Staib agar, Pal's agar and eggplant agar while 10 (52.6%) of them are positive with tobacco agar. C.neoformans colony forming unit (cfu) per plate were found as 51, 57 and 48 (median values) on simplified Staib agar, Pal's agar and eggplant agar, respectively, while tobacco agar has lower performance with 33 cfu/petri. No statistically significant difference were found between simplified Staib agar, Pal's agar and eggplant agar's performances for C.neoformans isolations from the nature (p=0.71). In conclusion, easily prepared eggplant agar is as functional as widely used media such as simplified Staib agar and Pal's agar for the isolation of C.neoformans from the natural environment.en_US
dc.language.isotren_US
dc.publisherAnkara Microbiology Societyen_US
dc.relation.ispartofMikrobiyoloji Bultenien_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCryptococcus neoformans: Eggplanten_US
dc.subjectEnvironmental isolationen_US
dc.subjectMediumen_US
dc.subjectPigmenten_US
dc.subjectSolanum melongenaen_US
dc.subjectagaren_US
dc.subjectarticleen_US
dc.subjectaubergineen_US
dc.subjectchemistryen_US
dc.subjectCryptococcus neoformansen_US
dc.subjectculture mediumen_US
dc.subjectevaluation studyen_US
dc.subjectgrowth, development and agingen_US
dc.subjectisolation and purificationen_US
dc.subjectmicrobiologyen_US
dc.subjectAgaren_US
dc.subjectCulture Mediaen_US
dc.subjectEnvironmental Microbiologyen_US
dc.titleEvaluation of a new medium, eggplant (Solanum melongena) agar as a screening medium for cryptococcus neoformans in environmental samplesen_US
dc.typeArticleen_US
dc.identifier.volume48en_US
dc.identifier.issue2en_US
dc.identifier.startpage292
dc.identifier.startpage292en_US
dc.identifier.endpage299en_US
dc.authorid0000-0003-2271-2618-
dc.authorid0000-0001-7783-8723-
dc.identifier.doi10.5578/mb.7450-
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.pmid24819266en_US
dc.identifier.scopus2-s2.0-84901472566en_US
dc.identifier.wosWOS:000336195500010en_US
dc.identifier.scopusqualityQ3-
dc.ownerPamukkale University-
item.cerifentitytypePublications-
item.grantfulltextnone-
item.openairetypeArticle-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.languageiso639-1tr-
item.fulltextNo Fulltext-
crisitem.author.dept14.03. Basic Medical Sciences-
crisitem.author.dept14.03. Basic Medical Sciences-
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Tıp Fakültesi Koleksiyonu
TR Dizin İndeksli Yayınlar Koleksiyonu / TR Dizin Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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