Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/7597
Title: Toxic effects of copper sulfate on the brains of term Hubbard broiler chicks: A stereological and biochemical study
Authors: Oguz, E.O.
Enli, Yaşar
Tufan, Ahmet Çevik
Turgut, G.
Keywords: Cavalieri
Chick brain
Copper sulfate
Development
Gluthatione
Malondialdehyde
copper sulfate
glutathione
malonaldehyde
animal
apoptosis
article
brain
brain level
chicken
drug effect
organ size
oxidation reduction reaction
oxidative stress
physiology
Animals
Apoptosis
Brain
Brain Chemistry
Chickens
Copper Sulfate
Glutathione
Organ Size
Oxidation-Reduction
Oxidative Stress
Abstract: Copper sulfate can cause different pathologies in different organ systems during development. We determined the effects of toxic levels of copper sulfate on brain development in term Hubbard broiler chicks using stereological and biochemical analyses. Hubbard broiler chicken eggs were divided into three groups: controls with no treatment, sham-treated animals and an experimental group. On day 1, 0.1 ml saline was injected into the air chambers of the sham and experimental groups. The experimental group received also 50 µg copper sulfate. At term (day 21), all chick brains were removed and their volumes were determined using the Cavalieri volume estimation. Parallel biochemical analyses were carried out for glutathione and malondialdehyde levels in the brain tissues as indicators of oxidative damage. With copper treatment, the mean brain volume (8079 µm3) was significantly decreased compared to both the control (10075 µm3) and sham (9547 µm3) groups. Copper treatment (143.8 nmol/g tissue) showed significantly decreased malondialdehyde levels compared to the control (293.6 nmol/g tissue) and sham groups (268.8 nmol/g tissue). Copper treatment (404.5 nmol/g tissue) showed significantly increased malondialdehyde levels compared to the control (158.6 nmol/g tissue) and sham (142.8 nmol/g tissue) groups. The morphological and biochemical parameters we measured demonstrated that in term Hubbard broiler chicks, toxic levels of copper sulfate cause developmental and oxidative brain damage. © 2013 The Biological Stain Commission.
URI: https://hdl.handle.net/11499/7597
https://doi.org/10.3109/10520295.2013.803601
ISSN: 1052-0295
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Tıp Fakültesi Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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