Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/7647
Title: Peptide-modified conducting polymer as a biofunctional surface: Monitoring of cell adhesion and proliferation
Authors: Oyman, G.
Geyik, C.
Ayranci, R.
Ak, Metin
Odaci Demirkol, D.
Timur, S.
Coskunol, H.
Keywords: Biofunctional
Publisher: Royal Society of Chemistry
Abstract: Here, we report the electropolymerization of 3-(2,5-di(thiophen-2-yl)-1H-pyrrol-1-yl)aniline monomer on indium tin oxide (ITO) glass and its use as a coating material for cell culture applications. Functional amino groups on the conducting polymer provide post-modification of the surface with the arginylglycylaspartic acid (RGD) peptide via EDC chemistry. Scanning electron microscopy, atomic force microscopy, and contact angle and surface conductivity measurements were carried out for the surface characterization. The peptide-conjugated surface was tested for adhesion and proliferation of several cell lines such as monkey kidney epithelial (Vero), human neuroblastoma (SH-SY5Y), and human immortalized skin keratinocyte (HaCaT). These cells were cultured on RGD-modified, polymer-coated ITO glass as well as conventional polystyrene surfaces for comparison. The data indicate that the RGD-modified surfaces exhibited better cell adhesion and proliferation among all surfaces compared. Cell imaging studies up to 72 h in length were performed on these surfaces using different microscopy techniques. Therefore, the novel biofunctional substrate is a promising candidate for further studies such as monitoring the effects of drugs and chemicals on cellular viability and morphology as well as cell-culture-on-a-chip applications. This journal is © the Partner Organisations 2014.
URI: https://hdl.handle.net/11499/7647
https://doi.org/10.1039/c4ra08481k
ISSN: 2046-2069
Appears in Collections:Fen-Edebiyat Fakültesi Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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