Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/7755
Title: Promoter hypermethylation-mediated down-regulation of RUNX3 gene in human brain tumors
Authors: Avci, C.B.
Dodurga, Yavuz
Susluer, S.Y.
Sigva, Z.O.D.
Yucebas, M.
Caglar, H.O.
Akalin, T.
Keywords: DNA methylation
Gene expression
Human brain tumors
RUNX3
Adult
Aged
Astrocytoma
Brain Neoplasms
Cell Line, Tumor
Core Binding Factor Alpha 3 Subunit
DNA Methylation
Down-Regulation
Female
Gene Expression Regulation, Neoplastic
Glioblastoma
Humans
Male
Meningeal Neoplasms
Meningioma
Middle Aged
Promoter Regions, Genetic
Reverse Transcriptase Polymerase Chain Reaction
RNA, Neoplasm
Young Adult
Publisher: Springer-Verlag London Ltd
Abstract: Background: The Runx family proteins, including RUNX3, are tissue-restricted transcription factors and play role in neuronal development and tumorigenesis. RUNX3 has an important role in glioblastoma (GBM) tumorigenesis because of its promoter hypermethylation. Aim: We aimed to evaluate the methylation-mediated expression regulation of RUNX3 gene in brain tumors. Patients and methods: Cases of meningiomas WHO grade III (3), anaplastic astrocytomas (3), diffuse astrocytoma (3), and GBM (12) were recruited into this study. Real-time quantitative PCR was performed for analyses of DNA promoter methylation and analyses of methylation-mediated expression status of RUNX3 gene was performed by real-time quantitative RT-PCR. Results: There was no significant difference between methylated and unmethylated quantitative ratio of RUNX3 gene promoter region and also no significant difference in relative ratio of RUNX3 gene expression in brain tumor groups. Methylated and unmethylated ratio in anaplastic astrocytoma, diffuse astrocytoma, GBM, meningioma (WHO grade III) and in all groups were; 1.44, 1.09, 1.51, 1.52 and 1.43, respectively. One allele was found methylated necessarily. No methylation was detected in one case of GBM group and one case of anaplastic astrocytoma group. There was no unmethylated promoter in one of the GBM cases. There were significant differences between relative ratio of RUNX3 gene expression and methylated/unmethylated ratio rates for all cases (p = 0.001) and GBM groups (p = 0.041). Conclusion: This study overemphasized the RUNX3 gene importance in brain tumors, due to the existence of at least one methylated allele. © Royal Academy of Medicine in Ireland 2013.
URI: https://hdl.handle.net/11499/7755
https://doi.org/10.1007/s11845-013-1001-3
ISSN: 0021-1265
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Tıp Fakültesi Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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