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https://hdl.handle.net/11499/7823
Title: | Investigation of conception rates achieved with the transfer of sexed and unsexed bovine embryo | Authors: | Karaşahin, Tahir Akyol, Numan Satılmış, Muharrem Kızıl, Sedat Hamdi Bucak, Mustafa Numan Çoyan, Kenan |
Keywords: | Bovine embryo sexing PCR Transfer Vitrification |
Publisher: | TUBITAK | Abstract: | The objective of this study was to investigate the conception rates achieved with the transfer of in-vivo-derived sexed and unsexed Holstein bovine embryos to appropriate recipients and to determine the accuracy of nonelectrophoretic PCR sexing. Sevenday- old embryos were derived in vivo by the nonsurgical flushing of the uterus. Before being vitrified and frozen some of the embryos obtained were sexed, while others were not sexed and were maintained as the control group. After thawing, the sexed and unsexed embryos were transferred to 23 and 21 bovine recipients, respectively. The conception rates achieved with the transfer of the sexed and unsexed embryos were 30.4% (7/23) and 42.9% (9/21), respectively. The difference between conception rates achieved in the 2 groups was not statistically significant (P > 0.05). For the sexed embryos the conception rates achieved with the transfer of male and female embryos were 27.2% (3/11) and 33.3% (4/12), respectively. The accuracy of embryo sexing with nonelectrophoretic PCR was 66.6% for male embryos and 100% for female embryos postdelivery. The mean rate of accuracy determined for embryo sexing at the end of the study was 83.33%. © TÜBİTAK. | URI: | https://hdl.handle.net/11499/7823 https://doi.org/10.3906/vet-1310-22 |
ISSN: | 1300-0128 |
Appears in Collections: | Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection Tıp Fakültesi Koleksiyonu TR Dizin İndeksli Yayınlar Koleksiyonu / TR Dizin Indexed Publications Collection WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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