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Title: | Regulation of URG4/URGCP and PPAR? gene expressions after retinoic acid treatment in neuroblastoma cells | Authors: | Avci, C.B. Dodurga, Yavuz Gündoğdu, Gülşah Caglar, H.O. Küçükatay, Vural Gündüz, C. Satıroğlu-Tufan, Naciye Lale |
Keywords: | Differentiation Neuroblastoma cells PPAR? SH-SY5Y URG4/URGCP glyceraldehyde 3 phosphate retinoic acid article cell differentiation controlled study GAPDH gene gene amplification gene expression regulation gene overexpression gene rearrangement human human cell metastasis neurite neuroblastoma cell oncogene polymerase chain reaction PPAR alpha gene priority journal protein binding protein phosphorylation reverse transcription polymerase chain reaction tumor recurrence URG4 URGCP gene |
Abstract: | Neuroblastoma (NB), originating from neural crest cells, is the most common extracranial tumor of childhood. Retinoic acid (RA) which is the biological active form of vitamin A regulates differentiation of NB cells, and RA derivatives have been used for NB treatment. PPAR? (peroxisome proliferator-activated receptor) plays an important role in the oxidation of fatty acids, carcinogenesis, and differentiation. URG4/URGCP gene is a proto-oncogene and that overexpression of URG4/URGCP is associated with metastasis and tumor recurrence in osteosarcoma. It has been known that URG4/URGCP gene is an overexpressed gene in hepatocellular carcinoma and gastric cancers. This study aims to detect gene expression patterns of PPAR? and URG4/URGCP genes in SH-SY5Y NB cell line after RA treatment. Expressions levels of PPAR? and URG4/URGCP genes were analyzed after RA treatment for reducing differentiation in SH-SY5Y NB cell line. To induce differentiation, the cells were treated with 10 µM RA in the dark for 3-10 days. Gene expression of URG4/URGCP and PPAR? genes were presented as the yield of polymerase chain reaction (PCR) products from target genes compared with the yield of PCR products from the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene. SH-SY5Y cells possess small processes in an undifferentiated state, and after treatment with RA, the cells developed long neurites, resembling a neuronal phenotype. PPAR? gene expression increased in RA-treated groups; URG4/URGCP gene expression decreased in SH-SY5Y cells after RA treatment compared with that in the control cells. NB cell differentiation might associate with PPAR? and URG4/URGCP gene expression profile after RA treatment. © 2013 International Society of Oncology and BioMarkers (ISOBM). | URI: | https://hdl.handle.net/11499/7893 https://doi.org/10.1007/s13277-013-0970-1 |
ISSN: | 1010-4283 |
Appears in Collections: | PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection Tıp Fakültesi Koleksiyonu WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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