Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/8024
Title: Glycohistochemistry of the lateral tympanic membrane in the syrinx of the denizli cock
Authors: Keskin, Nazan
İli, Pınar
Keywords: Denizli cock
Glycostructures
Syrinx
Tympanic membrane
Abstract: The Denizli cock is a special race that has harmonious long crowing. In this study, the syrinx of the Denizli cock was studied by using glycohistochemical analyses. Paraffin sections were stained with Alcian blue (AB) at pH 1 and pH 2.5 for demonstration of sulfated and carboxylated carbohydrates, respectively. At AB-pH 1, the lateral tympanic membrane (LTM) and also the medial tympanic membrane (MTM) exhibited a weak reactivity, whereas they were stained intensely at AB-pH 2.5. The frozen sections were incubated with 5 digoxigenin-labeled lectins for lectin histochemistry: peanut agglutinin, Maackia amurensis leucoagglutinin, Galanthus nivalis agglutinin, Datura stramonium agglutinin, and Sambucus nigra agglutinin. In LTM, positive stainings were observed for the 5 lectins. These results suggest that LTM exhibits an intense reactivity with AB-pH 2.5, indicating carboxylated carbohydrates, and it also has terminal saccharide moieties consisting of ß(1›3) linked galactose, ?(2›3) linked sialic acid, mannose, ß(1›4) linked galactose, and ?(2›6) linked sialic acid. © Tübİtak.
URI: https://hdl.handle.net/11499/8024
https://doi.org/10.3906/vet-1210-44
ISSN: 1300-0128
Appears in Collections:Fen-Edebiyat Fakültesi Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Tıp Fakültesi Koleksiyonu
TR Dizin İndeksli Yayınlar Koleksiyonu / TR Dizin Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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