Please use this identifier to cite or link to this item:
https://hdl.handle.net/11499/8053
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DC Field | Value | Language |
---|---|---|
dc.contributor.author | Bucak, M.N. | - |
dc.contributor.author | Keskin, Nazan | - |
dc.contributor.author | Taşpinar, M. | - |
dc.contributor.author | Çoyan, Kenan | - |
dc.contributor.author | Başpinar, N. | - |
dc.contributor.author | Cenariu, M.C. | - |
dc.contributor.author | Bilgili, A. | - |
dc.date.accessioned | 2019-08-16T12:34:52Z | |
dc.date.available | 2019-08-16T12:34:52Z | |
dc.date.issued | 2013 | - |
dc.identifier.issn | 0011-2240 | - |
dc.identifier.uri | https://hdl.handle.net/11499/8053 | - |
dc.identifier.uri | https://doi.org/10.1016/j.cryobiol.2013.04.007 | - |
dc.description.abstract | The aim of this study was to determine the effects of raffinose and hypotaurine on sperm parameters after the freeze-thawing of Merino ram sperm. Totally 40 ejaculates of five Merino ram were used in the study. Semen samples, which were diluted with a Tris-based extender containing 10. mM raffinose, 5. mM hypotaurine, 5. mM raffinose +2.5. mM hypotaurine (H + R) and no antioxidant (control), were cooled to 5 °C and frozen in 0.25. ml French straws and stored in liquid nitrogen. Frozen straws were then thawed individually at 37 °C for 25. s in a water bath for evaluation. The addition of raffinose led to higher percentages of subjective and CASA motilities (47.5 ± 12.2%, 46.3 ± 13.6%) compared to controls (38.8 ± 13.8%, 30.5 ± 11.7%, P< 0.05). For the CASA progressive motility, 5. mM raffinose (20.12 ± 8.82%) had increasing effect in comparison to control (10 ± 7.94%, P< 0.05) following the freeze-thawing process. Raffinose and hypotaurine led to higher viability (40.8 ± 4.68%, 40.8 ± 4.7%), high sperm mitochondrial activity (29.5 ± 5.4%, 27.3 ± 4.9%) and acrosome integrity (50.8 ± 8.1, 50.7 ± 4.4) percentages, compared to control groups (31.5 ± 3.5%, 9.5 ± 8.2%, 42.8 ± 7.3%, P< 0.05). H + R group only led to high sperm mitochondrial activity when compared to control group. In the comet test, raffinose and hypotaurine resulted in lower sperm with damaged DNA (6.2% and 3.9%) than that of control (9.1%), reducing the DNA damage. For TUNEL assay, The TUNEL-positive cell was distinguished by distinct nuclear staining. Raffinose and H + R groups resulted in lower sperm with TUNEL-positive cell (1.5 ± 1.2% and 2.1 ± 0.9%) than that of control (4.9 ± 2.5%) (P< 0.05). In conclusion, findings of this study showed that raffinose and hypotaurine supplementation in semen extenders provided a better protection of sperm parameters against cryopreservation injury, in comparison to the control groups. © 2013. | en_US |
dc.language.iso | en | en_US |
dc.relation.ispartof | Cryobiology | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | Additives | en_US |
dc.subject | Comet test | en_US |
dc.subject | Flourescent staining parameters | en_US |
dc.subject | Motility | en_US |
dc.subject | Ram sperm | en_US |
dc.subject | TUNEL-positive cells | en_US |
dc.subject | DNA | en_US |
dc.subject | hypotaurine | en_US |
dc.subject | raffinose | en_US |
dc.subject | acrosome | en_US |
dc.subject | animal cell | en_US |
dc.subject | article | en_US |
dc.subject | cell protection | en_US |
dc.subject | cell viability | en_US |
dc.subject | controlled study | en_US |
dc.subject | cryopreservation | en_US |
dc.subject | DNA damage | en_US |
dc.subject | freeze thawing | en_US |
dc.subject | male | en_US |
dc.subject | mitochondrion | en_US |
dc.subject | nonhuman | en_US |
dc.subject | priority journal | en_US |
dc.subject | sperm | en_US |
dc.subject | spermatozoon motility | en_US |
dc.subject | thawing | en_US |
dc.subject | Animals | en_US |
dc.subject | Cell Membrane | en_US |
dc.subject | Cryopreservation | en_US |
dc.subject | DNA Damage | en_US |
dc.subject | Male | en_US |
dc.subject | Mitochondria | en_US |
dc.subject | Raffinose | en_US |
dc.subject | Semen Preservation | en_US |
dc.subject | Sheep | en_US |
dc.subject | Sperm Motility | en_US |
dc.subject | Spermatozoa | en_US |
dc.subject | Taurine | en_US |
dc.title | Raffinose and hypotaurine improve the post-thawed Merino ram sperm parameters | en_US |
dc.type | Article | en_US |
dc.identifier.volume | 67 | en_US |
dc.identifier.issue | 1 | en_US |
dc.identifier.startpage | 34 | |
dc.identifier.startpage | 34 | en_US |
dc.identifier.endpage | 39 | en_US |
dc.identifier.doi | 10.1016/j.cryobiol.2013.04.007 | - |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.identifier.pmid | 23644017 | en_US |
dc.identifier.scopus | 2-s2.0-84879553329 | en_US |
dc.identifier.wos | WOS:000321601600006 | en_US |
dc.identifier.scopusquality | Q1 | - |
dc.owner | Pamukkale University | - |
item.grantfulltext | none | - |
item.openairetype | Article | - |
item.languageiso639-1 | en | - |
item.fulltext | No Fulltext | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
crisitem.author.dept | 14.03. Basic Medical Sciences | - |
crisitem.author.dept | 14.03. Basic Medical Sciences | - |
Appears in Collections: | PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection Tıp Fakültesi Koleksiyonu WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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