Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/8071
Title: A histochemical study of ultraviolet B irradiation and Origanum hypericifolium oil applied to the skin of mice
Authors: İli, Pınar
Keskin, Nazan
Keywords: Collagen fibers
Elastic fibers
Epidermal thickness
Origanum hypericifolium
Skin
UVB
vegetable oil
animal
article
Bagg albino mouse
chemistry
drug effect
female
mouse
Origanum
radiation exposure
skin
ultraviolet radiation
Animals
Female
Mice
Mice, Inbred BALB C
Plant Oils
Ultraviolet Rays
Lamiaceae
Mus
Abstract: Ultraviolet (UV) rays cause skin damage. Chronic exposure to UV irradiation causes decreased collagen synthesis, degenerative changes in collagen bundles, accumulation of elastotic material and increased epidermal thickness. Origanum hypericifolium, an endemic Turkish plant, belongs to Lamiaceae family. The main constituents of its oil are monoterpenes including cymene, carvacrol, thymol and ?-terpinene. The effects of undiluted O. hypericifolium oil on UVB irradiated skin of mice were investigated histochemically. Four groups of female BALB/c mice, whose dorsal hair was shaved, were allocated as follows: non-UVB irradiated (Group 1), UVB-irradiated (Group 2), O. hypericifolium oil treated (Group 3), and O. hypericifolium oil treated and UVB irradiated (Group 4). Sections of dorsal skin samples were stained with Mallory's phosphotungstic acid hematoxylin for collagen fibers and Taenzer-Unna orcein for elastic fibers. Sections also were stained with hematoxylin and eosin to measure epidermal thickness. We observed intense staining of collagen and homogeneous, scattered thin elastic fibers in Group 1; scattered and weakly stained collagen and curled, amorphous, accumulate elastic fibers in Group 2; and intense staining of collagen in Groups 3 and 4. Accumulation of elastic fibers in the dermis was unremarkable in Groups 3 and 4. In Groups 3 and 4, O. hypericifolium oil treatment thickened the epidermis. Epidermal thickness was greatest in Group 4. We suggest that O. hypericifolium oil may block UVB induced alterations of collagen and elastic fibers, and increase epidermal thickness. © 2013 The Biological Stain Commission.
URI: https://hdl.handle.net/11499/8071
https://doi.org/10.3109/10520295.2013.773077
ISSN: 1052-0295
Appears in Collections:Fen-Edebiyat Fakültesi Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Tıp Fakültesi Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

Show full item record



CORE Recommender

SCOPUSTM   
Citations

5
checked on Oct 13, 2024

WEB OF SCIENCETM
Citations

4
checked on Oct 31, 2024

Page view(s)

52
checked on Aug 24, 2024

Google ScholarTM

Check




Altmetric


Items in GCRIS Repository are protected by copyright, with all rights reserved, unless otherwise indicated.