Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/8162
Title: Leukemogenesis as a new approach to investigate the correlation between up regulated gene 4/upregulator of cell proliferation (URG4/URGCP) and signal transduction genes in leukemia
Authors: Dodurga, Yavuz
Oymak, Y.
Gündüz, C.
Şatıroğlu-Tufan, Naciye Lale
Vergin, C.
Çetingül, N.
Biray Avci, C.
Keywords: Acute leukemia
Cell cycle genes
Leukemogenesis
URG4/URGCP
complementary DNA
messenger RNA
acute granulocytic leukemia
acute lymphoblastic leukemia
adolescent
adult
article
ATM gene
cancer prognosis
CCNC gene
ccnd2 gene
CCNG1 gene
CCNG2 gene
CDC16 gene
CDK4 gene
CDK6 gene
CDK8 gene
CDKN2A gene
CDKN2D gene
CHEK1 gene
CHEK2 gene
child
clinical article
controlled study
correlation analysis
DNA synthesis
E2F4 gene
female
genetic association
genetic marker
human
human cell
infant
KRAS gene
leukemogenesis
male
oncogene
oncogene K ras
preschool child
quantitative analysis
real time polymerase chain reaction
RNA isolation
school child
signal transduction
tp53 gene
URG4 gene
URGCP gene
Adolescent
Cell Cycle Checkpoints
Cell Proliferation
Cell Transformation, Neoplastic
Child
Child, Preschool
Female
Gene Expression Regulation, Neoplastic
Humans
Infant
Infant, Newborn
Leukemia, Myeloid, Acute
Male
Neoplasm Proteins
Prognosis
Signal Transduction
Up-Regulation
Abstract: The aim of the study is to the determine the profiles of cell cycle genes and a new candidate oncogene of URG4/URGCP which play role in leukemia, establishing the association between the early prognosis of cancer and the quantitation of genetic changes, and bringing a molecular approach to definite diagnosis. In this study, 36 newly diagnosed patients' with ALL-AML in the range of 0-18 years and six control group patients' bone marrow samples were included. Total RNA was isolated from samples and then complementary DNA synthesis was performed. The obtained cDNAs have been installed 96 well plates after prepared appropriate mixtures and assessed with LightCycler ® 480 Real-Time PCR quantitatively. CHEK1, URG4/URGCP, CCNG1, CCNC, CDC16, KRAS, CDKN2D genes in the T-ALL group; CCND2, ATM, CDK8, CHEK1, TP53, CHEK2, CCNG2, CDK4, CDKN2A, E2F4, CCNC, KRAS genes in the precursor B-ALL group and CCND2, CDK6 genes in the AML group have shown significant increase in mRNA expression level. In the featured role of acute leukemia the regulating signaling pathways of leukemogenesis partially defined, although identification of new genetic markers in acute leukemia subgroups, will allow the development of early diagnostic and new treatment protocols. © 2012 Springer Science+Business Media Dordrecht.
URI: https://hdl.handle.net/11499/8162
https://doi.org/10.1007/s11033-012-2378-1
ISSN: 0301-4851
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Tıp Fakültesi Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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