Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/8394
Title: Effectiveness of Saccharomyces boulardii in a rat model of colitis
Authors: Soyturk, M.
Saygili, S.M.
Baskin, H.
Sagol, O.
Yılmaz, Osman
Saygılı, Fatih
Akpinar, H.
Keywords: Fungemia
Nitric oxide
Rat
Saccharomyces boulardii
Trinitrobenzene sulfonic acid
Tumor necrosis factor-a
methylprednisolone
nitric oxide
probiotic agent
tumor necrosis factor alpha
animal experiment
animal model
animal tissue
article
colitis
controlled study
defecation
disease activity
enzyme linked immunosorbent assay
female
fungemia
fungus identification
loose feces
nonhuman
rat
rectum hemorrhage
scoring system
weight reduction
Tumor necrosis factor-?
Animals
Anti-Inflammatory Agents
Colitis
Colon
Disease Models, Animal
Female
Gastrointestinal Agents
Inflammation Mediators
Methylprednisolone
Nitric Oxide
Probiotics
Rats
Rats, Wistar
Saccharomyces
Time Factors
Trinitrobenzenesulfonic Acid
Tumor Necrosis Factor-alpha
Abstract: AIM: To investigate the effects of Saccharomyces boulardii (S. boulardii in an experimental rat model of trinitrobenzene sulfonic acid (TNBS)-induced colitis. METHODS: Thirty-two Wistar albino female rats were categorized into five groups. On the first day of the study, 50 mg TNBS was administered via a rectal catheter in order to induce colitis in all rats, except those in the control group. For 14 d, the rats were fed a standard diet, without the administration of any additional supplements to either the control or TNBS groups, in addition to 1 mg/kg per day S. boulardii to the S. boulardii group, 1 mg/kg per day methyl prednisolone (MP) to the MP group. The animals in the S. boulardii + MP group were coadministered these doses of S. boulardii and MP. During the study, weight loss, stool consistency, and the presence of obvious blood in the stool were evaluated, and the disease activity index (DAI) for colitis was recorded. The intestines were examined and colitis was macro- and microscopically scored. The serum and tissue levels of tumor necrosis factor-? (TNF-?) and nitric oxide (NO) were determined, and fungemia was evaluated in the blood samples. RESULTS: The mean DAI scores for the MP and S. boulardii + MP groups was significantly lower than the TNBS group (3.69 ± 0.61 vs 4.46 ± 0.34, P = 0.018 and 3.77 ± 0.73 vs 4.46 ± 0.34, P = 0.025, respectively). While no significant differences between the TNBS and the S. boulardii or MP groups could be determined in terms of serum NO levels, the level of serum NO in the S. boulardii + MP group was significantly higher than in the TNBS and S. boulardii groups (8.12 ± 4.25 µmol/L vs 3.18 ± 1.19 µmol/L, P = 0.013; 8.12 ± 4.25 µmol/L vs 3.47 ± 1.66 µmol/L, P = 0.012, respectively). The tissue NO levels in the S. boulardii, MP and S. boulardii + MP groups were significantly lower than the TNBS group (16.62 ± 2.27 µmol/L vs 29.72 ± 6.10 µmol/L, P = 0.002; 14.66 ± 5.18 µmol/L vs 29.72 ± 6.10 µmol/L, P = 0.003; 11.95 ± 2.34 µmol/L vs 29.72 ± 6.10 µmol/L, P = 0.002, respectively). The tissue NO levels in the S. boulardii, MP and S. boulardii + MP groups were similar. The mean serum and tissue TNF-? levels were determined to be 12.97 ± 18.90 pg/mL and 21.75 ± 15.04 pg/mL in the control group, 18.25 ± 15.44 pg/mL and 25.27 ± 11.95 pg/mL in the TNBS group, 20.59 ± 16.15 pg/mL and 24.39 ± 13.06 pg/mL in the S. boulardii group, 9.05 ± 5.13 pg/mL and 24.46 ± 10.85 pg/mL in the MP group, and 13.95 ± 10.17 pg/mL and 24.26 ± 10.37 pg/mL in the S. boulardii + MP group. Significant differences in terms of the levels of serum and tissue TNF-? and the macroscopic and microscopic scores were not found between the groups. S. boulardii fungemia was not observed in any of the rats. However, Candida fungemia was detected in one rat (14%) in the TNBS group, two rats (28%) in the S. boulardii group, three rats (50%) in the MP group, and three rats (42%) in S. boulardii + MP group. CONCLUSION: S. boulardii does not demonstrate considerable effects on the DAI, pathological scores, or cytokine levels but does decrease the tissue NO levels. © 2012 Baishideng. All rights reserved.
URI: https://hdl.handle.net/11499/8394
https://doi.org/10.3748/wjg.v18.i44.6452
ISSN: 1007-9327
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Tıp Fakültesi Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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