Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/8398
Title: Methionine supplementation improves ram sperm parameters during liquid storage at 5°C
Authors: Bucak, M.N.
Çoyan, Kenan
Öztürk, C.
Güngör, Ş.
Ömür, A.D.
Keywords: Fluorescent staining
Lipoic acid
Liquid storage
Methionine
Ram sperm
methionine
thioctic acid
animal cell
article
cell viability
concentration (parameters)
controlled study
ejaculation
male
mitochondrion
nonhuman
priority journal
ram (sheep)
semen analysis
sperm preservation
spermatozoon motility
storage temperature
temperature dependence
Animals
Antioxidants
Cell Survival
Cold Temperature
Male
Semen Preservation
Sheep
Sperm Motility
Spermatozoa
Thioctic Acid
Abstract: The aim of this study was to investigate the effects of methionine and lipoic acid on ram sperm parameters during liquid storage (5 °C). Ejaculates collected from five Merino rams were pooled at 37 °C. Each pooled ejaculate was divided into five equal aliquots and diluted (37 °C) with five extenders, one of which was without additives, two of which contained methionine at two different doses, and the other two of which contained lipoic acid at two different doses. Sperm parameters were determined at 0, 24, 48, 72 and 96. h of liquid storage at 5 °C. The extenders containing 2 and 4. mM of methionine resulted in higher motility percentages, in comparison to the control, up to 96. h of storage. Methionine at doses of 2 and 4. mM led to higher viability and sperm mitochondrial activity percentages, when compared to the controls during 48, 72 and 96. h of liquid storage (P< 0.05). The findings of this study showed that methionine was of greater benefit to ram sperm parameters during liquid storage. © 2012.
URI: https://hdl.handle.net/11499/8398
https://doi.org/10.1016/j.cryobiol.2012.09.002
ISSN: 0011-2240
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Tıp Fakültesi Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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