Investigation of bartonella henselae antibodies in serum and plasma samples of kidney transplant patients

Abstract

Solid organ transplantation is an important therapeutic choice to improve the life quality of patients with end-stage renal disease. Renal transplant recipients have to take immunosuppressive therapy to prevent transplant rejection. However, this treatment increases susceptibility to infection. Bartonella henselae causes systemic, disseminated and silent manifestations in healthy individuals, while the mortality rate is high in immunosuppressive patients in the case of untreated bartonellosis. The diagnosis of B.henselae infections is usually based on serological methods since they are practical, simple and rapid. Recent reports indicated that bartonellosis seen after liver or kidney transplantation have been increased. The aim of this study was to present the antibody seropositivity of B.henselae detected in the serum and plasma samples of renal transplant recipients. This study was aimed to evaluate the antibody seroprevalence in renal transplant recipients and also to compare the antibody results obtained from serum and plasma samples. A total of 59 renal transplant recipients (32 male, 27 female; age range: 20-65 years) followed by Transplantation Unit of Health, Research and Training Center of Pamukkale University, were included in the study. After suspension of lyophilised B.henselae ATCC 49882 (Houston-1); B.henselae co-cultivation to Ve-ro cell culture was performed by the method recommended by Zbinden et al. [Clin Diagn Lab Immunol 1995; 2(6): 693-5]. The cells were taken to co-cultivation in flasks after development of monolayers. In house immunofluorescence antibody (IFA) method was performed with the use of infected cell-coated slides. B.henselae antibodies were studied at 1/64 screening dilution both in serum and plasma samples. In our study B.henselae antibody positivity rates found in serum and plasma samples of the patients were 16.9% (10/59) and 6.8% (4/59), respectively (Cohen k= 0.37). This detected kappa value indicated that the results of serum and plasma samples revealed "fair agreement". It should be kept in mind that the use of plasma samples in IFA tests would increase the false negative results. Thus the results of this study supported the general approach for the preference of serum samples for serological tests.