Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/8461
Title: Implication of C-type natriuretic peptide-3 signaling in glycosaminoglycan synthesis and chondrocyte hypertrophy during TGF-ß1 induced chondrogenic differentiation of chicken bone marrow-derived mesenchymal stem cells
Authors: Kocamaz, Erdoğan
Gök, Duygu
Çetinkaya, Ayşe
Tufan, Ahmet Çevik
Keywords: Chondrogenesis
Chondrogenic hypertrophy
CNP
Endochondral ossification
Glycosaminoglycan synthesis
NPR-B
alcian blue
C type natriuretic peptide 3
collagen type 2
collagen type 3
glycosaminoglycan
messenger RNA
natriuretic peptide receptor B
natriuretic peptide type C
transforming growth factor beta1
unclassified drug
animal cell
animal tissue
article
cartilage cell
cell culture
cell differentiation
chicken
chondrogenesis
controlled study
embryo
extracellular matrix
flow cytometry
gene expression
glycosaminoglycan metabolism
histology
hypertrophy
immunohistochemistry
mesenchymal stem cell
monolayer culture
nonhuman
pellet culture
priority journal
signal transduction
staining
Animals
Bone Marrow Cells
Cell Differentiation
Chick Embryo
Chickens
Chondrocytes
Collagen Type X
Glycosaminoglycans
Hypertrophy
Mesenchymal Stromal Cells
Natriuretic Peptide, C-Type
Receptors, Atrial Natriuretic Factor
Signal Transduction
Transforming Growth Factor beta1
Abstract: This study investigated the involvement of CNP-3, chick homologue for human C-type natriuretic peptide (CNP), in TGF-ß1 induced chondrogenic differentiation of chicken bone marrow-derived mesenchymal stem cells (MSCs). Chondrogenic differentiation of MSCs in pellet cultures was induced by TGF-ß1. Chondrogenic differentiation and glycosaminoglycan synthesis were analyzed on the basis of basic histology, collagen type II expression, and Alcian blue staining. Antibodies against CNP and NPR-B were used to block their function during these processes. Results revealed that expression of CNP-3 and NPR-B in MSCs were regulated by TGF-ß1 in monolayer cultures at mRNA level. In pellet cultures of MSCs, TGF-ß1 successfully induced chondrogenic differentiation and glycosaminoglycan synthesis. Addition of CNP into the TGF-ß1 supplemented chondrogenic differentiation medium further induced the glycosaminoglycan synthesis and hypertrophy of differentiated chondrocytes in these pellets. Pellets induced with TGF-b1 and treated with antibodies against CNP and NPR-B, did show collagen type II expression, however, Alcian blue staining showing glycosaminoglycan synthesis was significantly suppressed. In conclusion, CNP-3/NPR-B signaling may strongly be involved in synthesis of glycosaminoglycans of the chondrogenic matrix and hypertrophy of differentiated chondrocytes during TGF-ß1 induced chondrogenic differentiation of MSCs. © Springer Science+Business Media B.V. 2012.
URI: https://hdl.handle.net/11499/8461
https://doi.org/10.1007/s10735-012-9430-2
ISSN: 1567-2379
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Tıp Fakültesi Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

Show full item record



CORE Recommender

SCOPUSTM   
Citations

16
checked on Feb 24, 2024

WEB OF SCIENCETM
Citations

15
checked on Jun 7, 2024

Page view(s)

46
checked on May 27, 2024

Google ScholarTM

Check




Altmetric


Items in GCRIS Repository are protected by copyright, with all rights reserved, unless otherwise indicated.