Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/8716
Title: The expression of URGCP gene in prostate cancer cell lines: correlation with rapamycin
Authors: Dodurga, Yavuz
Avcı, Cağrı Biray
Süslüer, Sunde Yılmaz
Satıroglu Tufan, Naciye Lale
Gündüz, Cumhur
Keywords: antineoplastic antibiotic
rapamycin
tumor protein
URG4 protein, human
article
cell survival
drug effect
gene expression
gene expression regulation
genetics
human
IC 50
male
metabolism
prostate tumor
tumor cell line
Antibiotics, Antineoplastic
Cell Line, Tumor
Cell Survival
Gene Expression
Gene Expression Regulation, Neoplastic
Humans
Inhibitory Concentration 50
Male
Neoplasm Proteins
Prostatic Neoplasms
Sirolimus
Abstract: Molecular targets in prostate cancer are continually being explored, for which there are currently few therapeutic options. Rapamycin (RPM) is an antifungal macrolide antibiotic isolated from Streptomyces hygroscopicus which can inhibit the G1 to S transition. URGCP (upregulator of cell proliferation) is a novel gene located on chromosome 7p13. We aimed to investigate the role of URGCP gene expression changes in PC3, DU145, and LNCAP cell lines with/out RPM. Average cell viability and cytotoxic effect of rapamycin were investigated at 24 h intervals for three days by using Trypan blue dye exclusion test and XTT assay. Cytotoxic effects of rapamycin in DU145, PC3 and LNCAP cells were detected in time and dose dependent manner with the IC(50) doses within the range of 1-100 nM. As the results were evaluated, IC(50) doses in the DU145, PC3, and LNCaP cells were detected as 10, 25, and 50 nM, respectively. The mean relative ratios of URGCP gene expression in DU145, LNCAP and PC3 cells were found as -1.48, 6.59 and -13.00, respectively, when compared to rapamycin-free cells. The False Discovery Rate adjusted p value in DU145, LNCAP and PC3 were 1.25 × 10(-5), 2.20 × 10(-8) and 6.20 × 10(-9), respectively. When the URGCP gene expression level is compared between the dose and control group, we found that URGCP gene expression was significantly decreased in dose groups of DU145 and PC3 cells.
URI: https://hdl.handle.net/11499/8716
https://doi.org/10.1007/s11033-012-1891-6
ISBN: 15734978 (ISSN)
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Tıp Fakültesi Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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