Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/8780
Title: Expression of ERCC1 and its clinicopathological correlations in non-small cell lung cancer
Authors: Tepeli, Emre
Caner, Vildan
Büyükpınarbaşılı, N.
Çetin, Gökhan Ozan
Düzcan, Füsun
Elmas, Levent
Bağcı, Gülseren
Keywords: ERCC1
IHC
Non-small cell lung cancer
Real-time quantitative PCR
DNA
excision repair cross complementing protein 1
messenger RNA
aged
article
cancer staging
clinical feature
controlled study
DNA repair
down regulation
excision repair cross complementing group 1 gene
female
gene expression regulation
gene function
gene identification
genetic association
genetic marker
histopathology
human
human tissue
immunohistochemistry
lung non small cell cancer
major clinical study
male
molecular pathology
oncogene
protein expression
real time polymerase chain reaction
upregulation
validation process
Publisher: Kluwer Academic Publishers
Abstract: Excision Repair Cross-Complementing Group 1 (ERCC1) is an important DNA repair gene, playing critical role in nucleotide excision repair pathway and having a significant influence on genomic instability. Some studies support that ERCC1 might be a potential predictive and prognostic marker in non-small cell lung cancer (NSCLC). ERCC1 has also been shown to be a promising biomarker in NSCLC treated with a cisplatin-based regimen. Therefore, the determination of ERCC1 expression at DNA, mRNA and protein level in different stages of NSCLC is still an important topic in the cancer. Ninety-one formalin-fixed paraffin-embedded tumor samples histopathologically diagnosed as NSCLC were examined in this study. ERCC1 expression at protein level were scored by immunohistochemistry. The gene amplification and mRNA expression levels for ERCC1 were determined by real-time quantitative PCR. There was complete concordance among the three methods in 39 tumor samples (42.9%). A strong correlation was found between DNA amplification and mRNA expression (r = 0.662) while there was no correlation between mRNA and protein assessment for ERCC1 expression (r = -0.013). ERCC1 expression at mRNA and DNA level (63.1 and 84.2%, respectively) in tumors at stage III was higher than at the other stages. In contrast, the protein expression at stage II and III (56.6 and 52.6%, respectively) of NSCLC was lower than that of tumors with stage I NSCLC. These results show that the mechanism by which ERCC1 expression might play a role in tumor behavior. This study was also confirmed that the appropriate validation and qualification in methods used for ERCC1 status were needed before its clinical application and implementation. © 2011 Springer Science+Business Media B.V.
URI: https://hdl.handle.net/11499/8780
https://doi.org/10.1007/s11033-011-0743-0
ISSN: 0301-4851
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Tıp Fakültesi Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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