Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/8781
Title: The determination of relationship between "excision repair cross-complementing group 1" (ERCC1) gene T19007C and C8092A single nucleotide polymorphisms and clinicopathological parameters in non-small cell lung cancer
Authors: Koç, Esin
Caner, Vildan
Büyükpınarbaşılı, N.
Tepeli, Emre
Türk, Nilay Şen
Çetin, Gökhan Ozan
Bağcı, Gülseren
Keywords: ERCC1
Non-small cell lung cancer
Real-time PCR
Single nucleotide polymorphism
messenger RNA
adult
aged
article
cancer staging
clinical feature
controlled study
DNA extraction
DNA repair
excision repair cross complementing group 1 gene
female
gene frequency
gene function
genetic association
genetic variability
genotype
heterozygote
histopathology
human
human tissue
lung carcinogenesis
lung non small cell cancer
major clinical study
male
molecular pathology
oncogene
prediction
real time polymerase chain reaction
single nucleotide polymorphism
upregulation
Publisher: Kluwer Academic Publishers
Abstract: DNA repair plays a key role in prevention of carcinogenesis and one of the most important DNA repair mechanisms is nucleotide excision repair (NER) pathway. This pathway includes a number of genes such as excision repair cross-complementing group 1 (ERCC1) gene which are responsible for the 5' incision of damaged DNA. A reduced DNA repair capacity associated with ERCC1 mRNA level has been observed in lung carcinogenesis. Two single nucleotide polymorphisms (SNPs) in ERCC1 gene, T19007C (rs11615) and C8092A (rs3212986), reportedly predict to affect the mRNA of ERCC1 in non-small cell lung cancer (NSCLC). To examine the role of two common SNPs in ERCC1 gene further, we conducted this study where 80 cases histopatologically diagnosed as NSCLC were genotyped. Genomic DNA was extracted from formalin-fixed, paraffin embedded tissues and two SNPs were analyzed using real-time PCR. The distributions of TT, TC, and CC genotypes of the T19007C SNP were 40, 44 and 16%, respectively. Significantly increased frequency of the patients carrying at least one 19007C allele was observed in early stage compared to advanced stage (P = 0.002). And also, the frequency of TC and CC genotypes significantly increased in younger patients compared to older patients (P = 0.035). Regarding C8092A SNP, the distribution of CC, CA, and AA genotypes was 38, 51 and 11%, respectively. There was no significant difference in the genotype distribution between C8092A SNP and clinicopathological parameters. This study indicated that harboring at least one 19007C allele may have protective effect in NSCLC. © 2011 Springer Science+Business Media B.V.
URI: https://hdl.handle.net/11499/8781
https://doi.org/10.1007/s11033-011-0748-8
ISSN: 0301-4851
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Tıp Fakültesi Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

Show full item record



CORE Recommender

SCOPUSTM   
Citations

9
checked on Dec 7, 2024

WEB OF SCIENCETM
Citations

10
checked on Dec 11, 2024

Page view(s)

52
checked on Aug 24, 2024

Google ScholarTM

Check




Altmetric


Items in GCRIS Repository are protected by copyright, with all rights reserved, unless otherwise indicated.