Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/8789
Title: Investigation of microRNA expression changes in HepG2 cell line in presence of URG4/URGCP and in absence of URG4/URGCP suppressed by RNA interference
Authors: Dodurga, Yavuz
Yonguc, G.N.
Avci, C.B.
Bağcı, Gülseren
Gunduz, C.
Satıroğlu-Tufan, Naciye Lale
Keywords: Hepatocellular carcinoma cell line
miRNA
siRNA
URG4/URGCP
messenger RNA
microRNA
tumor protein
URG4 protein, human
article
cell strain HepG2
down regulation
gene expression profiling
gene expression regulation
genetics
human
metabolism
RNA interference
upregulation
Down-Regulation
Gene Expression Profiling
Gene Expression Regulation, Neoplastic
Hep G2 Cells
Humans
MicroRNAs
Neoplasm Proteins
RNA Interference
RNA, Messenger
Up-Regulation
Publisher: Springer Netherlands
Abstract: Hepatocellular carcinoma (HCC) originates from liver cells and is one of the most common malignant cancers in the world. microRNAs (miRNA), are single strand non-coding RNA molecules with the length of 18-25 nucleotides. miRNAs play an important role in the development of HCC, i.e., miRNAs have a significant impact on multistep hepatocellular carcinogenesis including cellular migration and invasion. URG4/URGCP (up-regulated gene-4/upregulator of cell proliferation) is up-regulated in the presence of HBxAg and has been identified and characterized by Satiroglu-Tufan et al. The full-length URG4/URGCP is 3.607 kb. Overexpression of URG4/URGCP in the presence of HBV X protein may function as a putative oncogene that significantly contributes to multi-step hepatocarcinogenesis. In this study, we aimed to investigate potential miRNA expression changes in HepG2 cell line model system in the presence of URG4/URGCP and in the absence of URG4/URGCP, which was suppressed by RNA interference. To functionally characterize URG4/URGCP, independent cultures of HepG2 cells were stably transfected with pcDNA3 or pcDNA3-URG4/URGCP. Relative quantification of whole genome miRNAs was analyzed by RT-PCR using human whole genome miRNA qPCR profiling kits. Among the 1,034 human miRNAs investigated by the arrays, 77 miRNAs were up-regulated and nine miRNAs were down-regulated in the presence of URG4/URGCP. In conclusion, we have analyzed miRNA profiles in HepG2 cells in presence or absence of URG4/URGCP gene using RNA interference. Some of these miRNAs may play roles in URG4/URGCP gene related disease development through the regulation of different signaling pathways. © 2012 Springer Science+Business Media Dordrecht.
URI: https://hdl.handle.net/11499/8789
https://doi.org/10.1007/s11033-012-2019-8
ISSN: 0301-4851
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Tıp Fakültesi Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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