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https://hdl.handle.net/11499/8789
Title: | Investigation of microRNA expression changes in HepG2 cell line in presence of URG4/URGCP and in absence of URG4/URGCP suppressed by RNA interference | Authors: | Dodurga, Yavuz Yonguc, G.N. Avci, C.B. Bağcı, Gülseren Gunduz, C. Satıroğlu-Tufan, Naciye Lale |
Keywords: | Hepatocellular carcinoma cell line miRNA siRNA URG4/URGCP messenger RNA microRNA tumor protein URG4 protein, human article cell strain HepG2 down regulation gene expression profiling gene expression regulation genetics human metabolism RNA interference upregulation Down-Regulation Gene Expression Profiling Gene Expression Regulation, Neoplastic Hep G2 Cells Humans MicroRNAs Neoplasm Proteins RNA Interference RNA, Messenger Up-Regulation |
Publisher: | Springer Netherlands | Abstract: | Hepatocellular carcinoma (HCC) originates from liver cells and is one of the most common malignant cancers in the world. microRNAs (miRNA), are single strand non-coding RNA molecules with the length of 18-25 nucleotides. miRNAs play an important role in the development of HCC, i.e., miRNAs have a significant impact on multistep hepatocellular carcinogenesis including cellular migration and invasion. URG4/URGCP (up-regulated gene-4/upregulator of cell proliferation) is up-regulated in the presence of HBxAg and has been identified and characterized by Satiroglu-Tufan et al. The full-length URG4/URGCP is 3.607 kb. Overexpression of URG4/URGCP in the presence of HBV X protein may function as a putative oncogene that significantly contributes to multi-step hepatocarcinogenesis. In this study, we aimed to investigate potential miRNA expression changes in HepG2 cell line model system in the presence of URG4/URGCP and in the absence of URG4/URGCP, which was suppressed by RNA interference. To functionally characterize URG4/URGCP, independent cultures of HepG2 cells were stably transfected with pcDNA3 or pcDNA3-URG4/URGCP. Relative quantification of whole genome miRNAs was analyzed by RT-PCR using human whole genome miRNA qPCR profiling kits. Among the 1,034 human miRNAs investigated by the arrays, 77 miRNAs were up-regulated and nine miRNAs were down-regulated in the presence of URG4/URGCP. In conclusion, we have analyzed miRNA profiles in HepG2 cells in presence or absence of URG4/URGCP gene using RNA interference. Some of these miRNAs may play roles in URG4/URGCP gene related disease development through the regulation of different signaling pathways. © 2012 Springer Science+Business Media Dordrecht. | URI: | https://hdl.handle.net/11499/8789 https://doi.org/10.1007/s11033-012-2019-8 |
ISSN: | 0301-4851 |
Appears in Collections: | PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection Tıp Fakültesi Koleksiyonu WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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