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https://hdl.handle.net/11499/9019
Title: | Design of a Lentiviral Vector for the Inducible Expression of MYC: A New Strategy for Construction Approach | Authors: | Tokgün, Onur Fiorentino, F.P. Tokgun, P.E. Yokota, J. Akça, Hakan |
Keywords: | Dox Inducible vector Lentivirus MCS Myc SCLC Cell culture Cells Cytology Electrophoresis Genes Genetic engineering Polymerase chain reaction Vectors Construction approaches Gene expression studies Inducible expression Inducible gene expression Small cell lung cancers Western-blot analysis Gene expression lentivirus vector Myc protein complementary DNA Article cancer cell controlled study DNA flanking region expression vector human human cell protein expression protein function real time polymerase chain reaction signal transduction small cell lung cancer Western blotting gene vector genetics tumor cell line Blotting, Western Cell Line, Tumor DNA, Complementary Genetic Vectors Humans Real-Time Polymerase Chain Reaction |
Publisher: | Humana Press Inc. | Abstract: | Lentiviral vectors are powerful tools for gene expression studies. Here we report the construction of pTIJ, a vector for inducible gene expression. pTIJ was generated from pTRIPZ backbone, which is designed for the inducible expression of shRNA sequences, by the introducing of a multiple cloning site upstream of the Tet promoter and the removal of miR30 flanking sequences. To evaluate pTIJ as a tool for the inducible expression of genes of interest, we introduced MYC cDNA into pTIJ and infected two small cell lung cancer cell lines, H209 and H345. Induction of MYC expression by doxycycline was detectable in both cell lines by real-time PCR and western blot analysis. This study highlights the relevance of pTIJ vector to allow the inducible expression of any gene of interest. In our belief, pTIJ will be an extremely useful tool to simplify the generation of genetically engineered cell lines for the inducible expression of cDNA sequences in biological studies. Furthermore, we report the generation of a pTIJ-MYC vector for the inducible expression of the oncogene MYC. © 2017, Springer Science+Business Media New York. | URI: | https://hdl.handle.net/11499/9019 https://doi.org/10.1007/s12033-017-0006-y |
ISSN: | 1073-6085 |
Appears in Collections: | PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection Tıp Fakültesi Koleksiyonu WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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