Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/9019
Title: Design of a Lentiviral Vector for the Inducible Expression of MYC: A New Strategy for Construction Approach
Authors: Tokgün, Onur
Fiorentino, F.P.
Tokgun, P.E.
Yokota, J.
Akça, Hakan
Keywords: Dox
Inducible vector
Lentivirus
MCS
Myc
SCLC
Cell culture
Cells
Cytology
Electrophoresis
Genes
Genetic engineering
Polymerase chain reaction
Vectors
Construction approaches
Gene expression studies
Inducible expression
Inducible gene expression
Small cell lung cancers
Western-blot analysis
Gene expression
lentivirus vector
Myc protein
complementary DNA
Article
cancer cell
controlled study
DNA flanking region
expression vector
human
human cell
protein expression
protein function
real time polymerase chain reaction
signal transduction
small cell lung cancer
Western blotting
gene vector
genetics
tumor cell line
Blotting, Western
Cell Line, Tumor
DNA, Complementary
Genetic Vectors
Humans
Real-Time Polymerase Chain Reaction
Publisher: Humana Press Inc.
Abstract: Lentiviral vectors are powerful tools for gene expression studies. Here we report the construction of pTIJ, a vector for inducible gene expression. pTIJ was generated from pTRIPZ backbone, which is designed for the inducible expression of shRNA sequences, by the introducing of a multiple cloning site upstream of the Tet promoter and the removal of miR30 flanking sequences. To evaluate pTIJ as a tool for the inducible expression of genes of interest, we introduced MYC cDNA into pTIJ and infected two small cell lung cancer cell lines, H209 and H345. Induction of MYC expression by doxycycline was detectable in both cell lines by real-time PCR and western blot analysis. This study highlights the relevance of pTIJ vector to allow the inducible expression of any gene of interest. In our belief, pTIJ will be an extremely useful tool to simplify the generation of genetically engineered cell lines for the inducible expression of cDNA sequences in biological studies. Furthermore, we report the generation of a pTIJ-MYC vector for the inducible expression of the oncogene MYC. © 2017, Springer Science+Business Media New York.
URI: https://hdl.handle.net/11499/9019
https://doi.org/10.1007/s12033-017-0006-y
ISSN: 1073-6085
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Tıp Fakültesi Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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