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https://hdl.handle.net/11499/9141
Title: | Effects of umbilical cord blood stem cells on healing factors for diabetic foot injuries | Authors: | Çil, Nazlı Oğuz, Emin Oğuzhan Mete, Ergün Çetinkaya, Ayşe Mete, Gülçin |
Keywords: | apoptosis VEGF Caspase 3 CD34 diabetic foot FGF IL-1 PDGF TGF-? TNF-ß wound healing biological marker CD34 antigen angiogenesis animal cytology experimental diabetes mellitus fetus blood gene expression regulation human physiology randomization rat stem cell stem cell transplantation Animals Antigens, CD34 Biomarkers Diabetes Mellitus, Experimental Diabetic Foot Fetal Blood Gene Expression Regulation Humans Neovascularization, Physiologic Random Allocation Rats Stem Cell Transplantation Stem Cells Wound Healing |
Publisher: | Taylor and Francis Ltd | Abstract: | The use of stem or progenitor cells from bone marrow, or peripheral or umbilical cord blood is becoming more common for treatment of diabetic foot problems. These cells promote neovascularization by angiogenic factors and they promote epithelium formation by stimulating cell replication and migration under certain pathological conditions. We investigated the role of CD34 + stem cells from human umbilical cord blood in wound healing using a rat model. Rats were randomly divided into a control group and two groups with diabetes induced by a single dose of 55 mg/kg intraperitoneal streptozocin. Scarred areas 5 mm in diameter were created on the feet of all rats. The diabetic rats constituted the diabetes control group and a diabetes + stem cell group with local injection into the wound site of 0.5 × 106 CD34 + stem cells from human umbilical cord blood. The newly formed skin in the foot wounds following CD34 + stem cell treatment showed significantly improvement by immunohistochemistry and TUNEL staining, and were closer to the wound healing of the control group than the untreated diabetic animals. The increase in FGF expression that accompanied the local injection of CD34 + stem cells indicates that FGF stimulation helped prevent apoptosis. Our findings suggest a promising new treatment approach to diabetic wound healing. © 2017 The Biological Stain Commission. | URI: | https://hdl.handle.net/11499/9141 https://doi.org/10.1080/10520295.2016.1243728 |
ISSN: | 1052-0295 |
Appears in Collections: | PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection Tıp Fakültesi Koleksiyonu WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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