Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/9141
Title: Effects of umbilical cord blood stem cells on healing factors for diabetic foot injuries
Authors: Çil, Nazlı
Oğuz, Emin Oğuzhan
Mete, Ergün
Çetinkaya, Ayşe
Mete, Gülçin
Keywords: apoptosis VEGF
Caspase 3
CD34
diabetic foot
FGF
IL-1
PDGF
TGF-?
TNF-ß
wound healing
biological marker
CD34 antigen
angiogenesis
animal
cytology
experimental diabetes mellitus
fetus blood
gene expression regulation
human
physiology
randomization
rat
stem cell
stem cell transplantation
Animals
Antigens, CD34
Biomarkers
Diabetes Mellitus, Experimental
Diabetic Foot
Fetal Blood
Gene Expression Regulation
Humans
Neovascularization, Physiologic
Random Allocation
Rats
Stem Cell Transplantation
Stem Cells
Wound Healing
Publisher: Taylor and Francis Ltd
Abstract: The use of stem or progenitor cells from bone marrow, or peripheral or umbilical cord blood is becoming more common for treatment of diabetic foot problems. These cells promote neovascularization by angiogenic factors and they promote epithelium formation by stimulating cell replication and migration under certain pathological conditions. We investigated the role of CD34 + stem cells from human umbilical cord blood in wound healing using a rat model. Rats were randomly divided into a control group and two groups with diabetes induced by a single dose of 55 mg/kg intraperitoneal streptozocin. Scarred areas 5 mm in diameter were created on the feet of all rats. The diabetic rats constituted the diabetes control group and a diabetes + stem cell group with local injection into the wound site of 0.5 × 106 CD34 + stem cells from human umbilical cord blood. The newly formed skin in the foot wounds following CD34 + stem cell treatment showed significantly improvement by immunohistochemistry and TUNEL staining, and were closer to the wound healing of the control group than the untreated diabetic animals. The increase in FGF expression that accompanied the local injection of CD34 + stem cells indicates that FGF stimulation helped prevent apoptosis. Our findings suggest a promising new treatment approach to diabetic wound healing. © 2017 The Biological Stain Commission.
URI: https://hdl.handle.net/11499/9141
https://doi.org/10.1080/10520295.2016.1243728
ISSN: 1052-0295
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Tıp Fakültesi Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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