Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/9196
Title: Bacteria induced extrinsic and intrinsic apoptotic pathways in the rat gastrointestinal system
Authors: Mete, Ergün
Cevahir, Nural
Oğuz, Emin Oğuzhan
Şahin, Barbaros
Kaleli, İlknur
Özdemir, Mehmet Bülent
Köseler, Aylin
Keywords: Apoptosis
Bacteria translocation
Caspases
P53
Pathway
Sepsis
caspase 3
caspase 8
caspase 9
protein p53
animal cell
animal tissue
aorta clamping
apoptosis
Article
bacterial translocation
bacterium colony
bacterium culture
colon
controlled study
Escherichia coli
gastrointestinal tract
histopathology
immunohistochemistry
inoculation
Klebsiella
liver
mesentery lymph node
nonhuman
protein expression
protein function
protein localization
rat
signal transduction
small intestine
spleen
staining
Publisher: Scientific Publishers of India
Abstract: Apoptosis is a key factor in the death of organ-specific cells. Developing a clear understanding of the effect that bacterial translocation has on initiating apoptotic pathways that induce multiple organ dysfunction syndrome (MODS) is essential for developing effective treatment modalities. Translocation does not occur naturally and apoptotic pathways remain unclear. The aim of this study is to investigate the effect of bacterial translocation on apoptotic pathways in the spleen, small intestine, colon, liver, and mesenteric lymph nodes (MLN) of rats. We randomly divided 12 healthy Wistar-Albino rats into two groups and induced bacterial translocation in the experiment group by clamping the superior mesenteric arteries (SMA) for comparison of test results to the control group. Samples from both groups were collected under sterile conditions and an inoculation procedure was performed. Caspase 3, 8, 9 and p53 were evaluated by immunohistochemistry, and cell expressions were counted. Klebsiella and E. coli colonies were observed in the bacteria cultures associated with the experiment group. Bacterial translocation-activated caspase 8 and 3 were found in all tissues of the experiment group; however, activated p53 was identified only in the colon and liver and activated caspase 9 was seen in small intestine, mesenteric lymph nodes and liver. We concluded that translocated bacteria stimulated extrinsic and intrinsic signaling pathway in gastrointestinal systems. © 2017, Scientific Publishers of India. All rights reserved.
URI: https://hdl.handle.net/11499/9196
ISSN: 0970-938X
Appears in Collections:Acıpayam Meslek Yüksekokulu Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Tıp Fakültesi Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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