Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/9413
Title: Tissue reaction to urogynecologic meshes: effect of steroid soaking in two different mesh models
Authors: Karabulut, Aysun
Simavlı, Serap Aynur
Abban, Gülçin Mete
Akyer, Şahika Pınar
Keskin, Nazan
Tan, Semih
Şahin, Barbaros
Keywords: Inflammation
Mesh
Polypropylene
Polyvinylidene fluoride
Tissue reaction
Urogynecology
CD15 antigen
CD3 antibody
CD34 antibody
CD68 antigen
collagen
polypropylene
polyvinylidene fluoride
steroid
polyvinyl derivative
animal cell
animal experiment
animal tissue
Article
cell ultrastructure
controlled study
electron microscopy
giant cell
histology
immunohistochemistry
inflammation
lymphocyte
macrophage
mesh sling
neutrophil
nonhuman
priority journal
protein expression
rat
rectus abdominis muscle
staining
stem cell
tissue reaction
abdominal wall
adverse effects
animal
comparative study
diagnostic imaging
female
Foreign-Body Reaction
human
materials testing
metabolism
surgical mesh
Wistar rat
Abdominal Wall
Animals
Collagen
Female
Humans
Materials Testing
Polypropylenes
Polyvinyls
Rats
Rats, Wistar
Steroids
Surgical Mesh
Publisher: Springer London
Abstract: Introduction and Hypothesis: Steroid soaking may decrease mesh-triggered inflammatory reaction in tissue. We aimed to investigate the tissue reaction to a steroid-soaked mesh material and an unsoaked mesh material in the rat model. Methods: Neutral and steroid-soaked type I macroporous polypropylene (PP) monofilament and polyvinylidene fluoride (PVF) mesh materials were implanted on the rectus abdominis muscle of 20 mature Wistar albino rats. Animals were divided into four groups: PP mesh with steroid (PP-S), PP mesh without steroid, PVF mesh with steroid (PVF-S), and PVF mesh without steroid. The rats were killed after 12 weeks, and histologic, immunohistochemical and electron microscopic examinations were performed. For immunohistochemical analysis, polyclonal rabbit anti-mouse CD3, rabbit anti-mouse CD68, rabbit anti-mouse CD15, and rabbit anti-mouse CD34 antibodies were used for the detection of lymphocytes, macrophages, polymorphonuclear leukocyte foreign body giant cells, and fibromyocyte stem cells, respectively. Samples were stained with hematoxylin and eosin for the histologic evaluation of inflammation and with Masson’s trichrome stain for the evaluation of collagen deposition. Pore size and mesh ultrastructure were evaluated by electron microscopy. Results: Expression of CD3 was lower in the PVF, PVF-S and PP-S groups, and expression of CD34 was higher in the PVF-S and PP-S groups than in the PP groups (p < 0.05). Collagen deposition was lower in the PVF, PVF-S and PP-S groups (p < 0.05). Histologically, the intensity of inflammation was lower in the PVF-S and PP-S groups than in the PP mesh group (p < 0.05). There were no significant differences among the groups in terms of pore size and mesh ultrastructure on electron microscopic examination (p > 0.05). Conclusions: PVF mesh induces less inflammation than PP mesh, and in both mesh types steroid soaking further decreases inflammation without changing the pore size. © 2016, The International Urogynecological Association.
URI: https://hdl.handle.net/11499/9413
https://doi.org/10.1007/s00192-016-3013-9
ISSN: 0937-3462
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Tıp Fakültesi Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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