Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/9414
Title: Extract of Calvatia gigantea inhibits proliferation of A549 human lung cancer cells
Authors: Eroğlu, Canan
Seçme, Mücahit
Atmaca, Pelin
Kaygusuz, Oğuzhan
Gezer, Kudret
Bağcı, Gülseren
Dodurga, Yavuz
Keywords: Apoptosis
Calvatia gigantea
Cell cycle
Lung cancer cell line
2 3 bis (2 methoxy 4 nitro 5 sulfophenyl) 2h tetrazolium 5 carboxanilide
antineoplastic agent
Calvatia gigantea extract
caspase 3
caspase 9
cyclin D1
cyclin D2
cyclin dependent kinase 4
plant extract
protein Bax
protein bcl 2
protein kinase B
protein p21
protein p53
tetrazole derivative
unclassified drug
A549 cell line
antineoplastic activity
antiproliferative activity
apoptosis
Article
cell cycle arrest
cell proliferation
cell viability
controlled study
cytotoxicity
cytotoxicity assay
down regulation
edible mushroom
human
human cell
human cell culture
IC50
in vitro study
medicinal plant
mushroom
protein expression
XTT assay
Publisher: Springer Netherlands
Abstract: In this study, in order to investigate the anticancer mechanism of Calvatia gigantea extract, edible mushroom species, which belong to Lycoperdaceae family, changes of CCND1, CCND2, CDK4, p21, Akt, Bax, Bcl-2, p53, caspase-3 and caspase-9 were evaluated in A549 lung cancer cells. Cytotoxic effect of C.gigantea extract was evaluated by using XTT (2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5 carboxanilide). The C. gigantea extract was treated in a time and dose dependent manner within the range 25 µg/ml–2 mg/ml to determine the IC50 dose. IC50 dose for C. gigantea extract was detected as 500 µg/ml for 72 h. According to expression results, while CCND1, CCND2, CDK4, Akt and Bcl-2 expression clearly decreased, Bax, p53, caspase-3 and caspase-9 expression clearly increased in the dose group cells (A549 cells treated with 500 µg/ml dose of C. gigantea extract for 72 h). However, there was no change in p21 expression. C. gigantea extract induced cell cycle arrest and apoptosis by decreasing the CCND1, CCND2, CDK4, Akt and Bcl-2 expression and by increasing Bax, p53, caspase-3 and caspase-9 expression in A549 cells. Mushrooms are eukaryotic organisms heavily used because of their supposedly anticancer effect. Many mushroom species have been used for medical purposes, as a result of also having many effects such as antibiotic, antiviral and anticancer effects. It is thought that the C. gigantea extract may be a significant agent for treatment of lung cancer as a single agent or in combination with other drugs. © 2016, Springer Science+Business Media Dordrecht.
URI: https://hdl.handle.net/11499/9414
https://doi.org/10.1007/s10616-016-9947-4
ISSN: 0920-9069
Appears in Collections:Fen-Edebiyat Fakültesi Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Tıp Fakültesi Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

Files in This Item:
File SizeFormat 
10.1007 s10616-016-9947-4.pdf648.85 kBAdobe PDFView/Open
Show full item record



CORE Recommender

SCOPUSTM   
Citations

24
checked on Dec 7, 2024

WEB OF SCIENCETM
Citations

20
checked on Dec 11, 2024

Page view(s)

84
checked on Aug 24, 2024

Download(s)

36
checked on Aug 24, 2024

Google ScholarTM

Check




Altmetric


Items in GCRIS Repository are protected by copyright, with all rights reserved, unless otherwise indicated.