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Title: | Extract of Calvatia gigantea inhibits proliferation of A549 human lung cancer cells | Authors: | Eroğlu, Canan Seçme, Mücahit Atmaca, Pelin Kaygusuz, Oğuzhan Gezer, Kudret Bağcı, Gülseren Dodurga, Yavuz |
Keywords: | Apoptosis Calvatia gigantea Cell cycle Lung cancer cell line 2 3 bis (2 methoxy 4 nitro 5 sulfophenyl) 2h tetrazolium 5 carboxanilide antineoplastic agent Calvatia gigantea extract caspase 3 caspase 9 cyclin D1 cyclin D2 cyclin dependent kinase 4 plant extract protein Bax protein bcl 2 protein kinase B protein p21 protein p53 tetrazole derivative unclassified drug A549 cell line antineoplastic activity antiproliferative activity apoptosis Article cell cycle arrest cell proliferation cell viability controlled study cytotoxicity cytotoxicity assay down regulation edible mushroom human human cell human cell culture IC50 in vitro study medicinal plant mushroom protein expression XTT assay |
Publisher: | Springer Netherlands | Abstract: | In this study, in order to investigate the anticancer mechanism of Calvatia gigantea extract, edible mushroom species, which belong to Lycoperdaceae family, changes of CCND1, CCND2, CDK4, p21, Akt, Bax, Bcl-2, p53, caspase-3 and caspase-9 were evaluated in A549 lung cancer cells. Cytotoxic effect of C.gigantea extract was evaluated by using XTT (2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5 carboxanilide). The C. gigantea extract was treated in a time and dose dependent manner within the range 25 µg/ml–2 mg/ml to determine the IC50 dose. IC50 dose for C. gigantea extract was detected as 500 µg/ml for 72 h. According to expression results, while CCND1, CCND2, CDK4, Akt and Bcl-2 expression clearly decreased, Bax, p53, caspase-3 and caspase-9 expression clearly increased in the dose group cells (A549 cells treated with 500 µg/ml dose of C. gigantea extract for 72 h). However, there was no change in p21 expression. C. gigantea extract induced cell cycle arrest and apoptosis by decreasing the CCND1, CCND2, CDK4, Akt and Bcl-2 expression and by increasing Bax, p53, caspase-3 and caspase-9 expression in A549 cells. Mushrooms are eukaryotic organisms heavily used because of their supposedly anticancer effect. Many mushroom species have been used for medical purposes, as a result of also having many effects such as antibiotic, antiviral and anticancer effects. It is thought that the C. gigantea extract may be a significant agent for treatment of lung cancer as a single agent or in combination with other drugs. © 2016, Springer Science+Business Media Dordrecht. | URI: | https://hdl.handle.net/11499/9414 https://doi.org/10.1007/s10616-016-9947-4 |
ISSN: | 0920-9069 |
Appears in Collections: | Fen-Edebiyat Fakültesi Koleksiyonu PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection Tıp Fakültesi Koleksiyonu WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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