Please use this identifier to cite or link to this item:
https://hdl.handle.net/11499/9504
Title: | Anti-tumor effects of bemiparin in HepG2 and MIA PaCa-2 cells | Authors: | Alur, İhsan Dodurga, Yavuz Seçme, Mücahit Elmas, Levent Bağcı, Gülseren Gökşin, İbrahim Avcı, Ç.B. |
Keywords: | Bemiparin HepG2 MIA PaCa-2 bemiparin caspase 3 caspase 8 caspase 9 cyclin D1 cyclin dependent kinase 4 cyclin dependent kinase 6 Fas associated death domain protein HLA DR4 antigen messenger RNA protein Bax protein bcl 2 protein Bid protein p16 protein p21 protein p53 tumor necrosis factor receptor associated death domain protein antineoplastic agent low molecular weight heparin antineoplastic activity Article colony formation controlled study gene expression hepatocellular carcinoma cell line HepG2 cell line human human cell IC50 Mia Paca 2 cancer cell line pancreas cancer priority journal real time polymerase chain reaction XTT assay apoptosis drug effects gene tumor cell line Antineoplastic Agents Apoptosis Cell Line, Tumor Genes, cdc Heparin, Low-Molecular-Weight Humans |
Publisher: | Elsevier B.V. | Abstract: | Recent researches have demonstrated improved survival in oncologic patients treated with low molecular weight heparins (LMWHs) which are anticoagulant drugs. We evaluated "second generation" LMWH bemiparin and its in vitro anti-tumor effects on HepG2 hepatocellular carcinoma and MIA PaCa-2 cancer cells. The aim of the study is to investigate anti-cancer mechanism of bemiparin in HepG2 and Mia-Paca-2 cancer cells. Cytotoxic effects of bemiparin were determined by XTT assay. IC50 dose of bemiparin was found to be 200 IU/mL in the 48th hour in the MiaPaCa-2 cell line and 50 IU/mL in the 48th hour in the HepG2 cell line. CCND1 (cyclin D1), CDK4, CDK6, p21, p16, p53, caspase-3, caspase-9, caspase-8, Bcl-2, BID, DR4, DR5, FADD, TRADD, Bax, gene mRNA expressions were evaluated by Real-time PCR. Real-time PCR analysis showed that CCND1 expression was reduced in HepG2 dose the group cells when compared with the control group cells and p53, caspase-3, caspase p21, caspase-8 and expressions were increased in the dose group cells when compared with the control group cells. CCND1, CDK4 and CDK6 expressions were reduced in MIA PaCa-2 dose group cells when compared with the control group cells and p53 expression was increased in the dose group cells when compared with the control group cells. Other expressions of genes were found statistically insignificant both of cell lines.It was found that bemiparin in HepG2 and MIA PaCa-2 cells suppressed invasion, migration, and colony formation by using matrigel invasion chamber, and colony formation assay, respectively. In conclusion, it is thought that bemiparin indicates anti-tumor activity by affecting cell cycle arrest, apoptosis, invasion, migration, and colony formation on cancer cells. © 2016 Elsevier B.V. | URI: | https://hdl.handle.net/11499/9504 https://doi.org/10.1016/j.gene.2016.03.044 |
ISSN: | 0378-1119 |
Appears in Collections: | PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection Tıp Fakültesi Koleksiyonu WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
Show full item record
CORE Recommender
SCOPUSTM
Citations
8
checked on Nov 30, 2024
WEB OF SCIENCETM
Citations
8
checked on Nov 29, 2024
Page view(s)
94
checked on Aug 24, 2024
Google ScholarTM
Check
Altmetric
Items in GCRIS Repository are protected by copyright, with all rights reserved, unless otherwise indicated.