Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/9504
Title: Anti-tumor effects of bemiparin in HepG2 and MIA PaCa-2 cells
Authors: Alur, İhsan
Dodurga, Yavuz
Seçme, Mücahit
Elmas, Levent
Bağcı, Gülseren
Gökşin, İbrahim
Avcı, Ç.B.
Keywords: Bemiparin
HepG2
MIA PaCa-2
bemiparin
caspase 3
caspase 8
caspase 9
cyclin D1
cyclin dependent kinase 4
cyclin dependent kinase 6
Fas associated death domain protein
HLA DR4 antigen
messenger RNA
protein Bax
protein bcl 2
protein Bid
protein p16
protein p21
protein p53
tumor necrosis factor receptor associated death domain protein
antineoplastic agent
low molecular weight heparin
antineoplastic activity
Article
colony formation
controlled study
gene expression
hepatocellular carcinoma cell line
HepG2 cell line
human
human cell
IC50
Mia Paca 2 cancer cell line
pancreas cancer
priority journal
real time polymerase chain reaction
XTT assay
apoptosis
drug effects
gene
tumor cell line
Antineoplastic Agents
Apoptosis
Cell Line, Tumor
Genes, cdc
Heparin, Low-Molecular-Weight
Humans
Publisher: Elsevier B.V.
Abstract: Recent researches have demonstrated improved survival in oncologic patients treated with low molecular weight heparins (LMWHs) which are anticoagulant drugs. We evaluated "second generation" LMWH bemiparin and its in vitro anti-tumor effects on HepG2 hepatocellular carcinoma and MIA PaCa-2 cancer cells. The aim of the study is to investigate anti-cancer mechanism of bemiparin in HepG2 and Mia-Paca-2 cancer cells. Cytotoxic effects of bemiparin were determined by XTT assay. IC50 dose of bemiparin was found to be 200 IU/mL in the 48th hour in the MiaPaCa-2 cell line and 50 IU/mL in the 48th hour in the HepG2 cell line. CCND1 (cyclin D1), CDK4, CDK6, p21, p16, p53, caspase-3, caspase-9, caspase-8, Bcl-2, BID, DR4, DR5, FADD, TRADD, Bax, gene mRNA expressions were evaluated by Real-time PCR. Real-time PCR analysis showed that CCND1 expression was reduced in HepG2 dose the group cells when compared with the control group cells and p53, caspase-3, caspase p21, caspase-8 and expressions were increased in the dose group cells when compared with the control group cells. CCND1, CDK4 and CDK6 expressions were reduced in MIA PaCa-2 dose group cells when compared with the control group cells and p53 expression was increased in the dose group cells when compared with the control group cells. Other expressions of genes were found statistically insignificant both of cell lines.It was found that bemiparin in HepG2 and MIA PaCa-2 cells suppressed invasion, migration, and colony formation by using matrigel invasion chamber, and colony formation assay, respectively. In conclusion, it is thought that bemiparin indicates anti-tumor activity by affecting cell cycle arrest, apoptosis, invasion, migration, and colony formation on cancer cells. © 2016 Elsevier B.V.
URI: https://hdl.handle.net/11499/9504
https://doi.org/10.1016/j.gene.2016.03.044
ISSN: 0378-1119
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Tıp Fakültesi Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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