Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/9523
Title: Investigation of anticancer mechanism of oleuropein via cell cycle and apoptotic pathways in SH-SY5Y neuroblastoma cells
Authors: Seçme, Mücahit
Eroğlu, C.
Dodurga, Yavuz
Bağcı, Gülseren
Keywords: Apoptosis
Cell cycle
Neuroblastoma
Oleuropein
caspase 3
caspase 9
cyclin D1
cyclin D2
cyclin D3
cyclin dependent kinase 4
cyclin dependent kinase inhibitor 1A
cyclin dependent kinase inhibitor 2B
oleuropein
protein Bax
protein p53
antineoplastic agent
antioxidant
iridoid
antineoplastic activity
antiproliferative activity
apoptosis
Article
cell cycle
cell cycle arrest
cell cycle regulation
cell invasion
cell invasion assay
cell migration
cell proliferation
cell viability
colony formation
controlled study
down regulation
human
human cell
IC50
in vitro study
neuroblastoma
neuroblastoma cell line
priority journal
protein expression
SH SY5Y neuroblastoma cell line
TUNEL assay
upregulation
Western blotting
wound healing
cell division
cell motion
cell survival
drug effects
G1 phase cell cycle checkpoint
gene expression regulation
pathology
tumor cell line
tumor invasion
Antineoplastic Agents
Antioxidants
Cell Division
Cell Line, Tumor
Cell Movement
Cell Proliferation
Cell Survival
G1 Phase Cell Cycle Checkpoints
Gene Expression Regulation, Neoplastic
Humans
In Situ Nick-End Labeling
Iridoids
Neoplasm Invasiveness
Wound Healing
Publisher: Elsevier B.V.
Abstract: Neuroblastoma is one of the most common types of pediatric tumors that can spread quickly in neuronal tissues. Oleuropein which is active compound of olive leaves, belongs to polyphenols group and has antioxidant, anti-microbial, anti-inflammatory, anti-hypertensive and anti-carcinogenic effects. The aim of the study is to determine the therapeutic effects of oleuropein on cell proliferation, invasion, colony formation, cell cycle and apoptotic mechanisms in SH-SY5Y neuroblastoma cell line under in vitro conditions. The effect of oleuropein on cell viability was determined by XTT method. 84 cell cycle control and 84 apoptosis related genes were evaluated by RT-PCR. Effects of oleuropein on apoptosis were researched by TUNEL assay. Protein expressions were determined by western blot analysis. Effects of oleuropein on cell invasion, colony formation and migration were detected by matrigel-chamber, colony formation assay and wound-healing assay, respectively. IC50 value of oleuropein in SH-SY5Y cells was detected as 350 µM at 48th hours. It is determined that oleuropein causes cell cycle arrest by down-regulating of CylinD1,CylinD2,CyclinD3,CDK4,CDK6 and up-regulating of p53 and CDKN2A, CDKN2B, CDKN1A gene expressions. Oleuropein also induces apoptosis by inhibiting of Bcl-2 and activating of Bax,caspase-9 and caspase-3 gene expressions. Apoptotic cell ratio was found 36.4 ± 3.27% in oleuropein dose group. Oleuropein decreased invasion in SH-SY5Y cells and suppressed colony numbers in ratio of 53.6 ± 4.71%.Our results demonstrated that oleuropein can be a therapeutic agent in the treatment of neuroblastoma. © 2016 Elsevier B.V.
URI: https://hdl.handle.net/11499/9523
https://doi.org/10.1016/j.gene.2016.03.038
ISSN: 0378-1119
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Tıp Fakültesi Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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