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https://hdl.handle.net/11499/9523
Title: | Investigation of anticancer mechanism of oleuropein via cell cycle and apoptotic pathways in SH-SY5Y neuroblastoma cells | Authors: | Seçme, Mücahit Eroğlu, C. Dodurga, Yavuz Bağcı, Gülseren |
Keywords: | Apoptosis Cell cycle Neuroblastoma Oleuropein caspase 3 caspase 9 cyclin D1 cyclin D2 cyclin D3 cyclin dependent kinase 4 cyclin dependent kinase inhibitor 1A cyclin dependent kinase inhibitor 2B oleuropein protein Bax protein p53 antineoplastic agent antioxidant iridoid antineoplastic activity antiproliferative activity apoptosis Article cell cycle cell cycle arrest cell cycle regulation cell invasion cell invasion assay cell migration cell proliferation cell viability colony formation controlled study down regulation human human cell IC50 in vitro study neuroblastoma neuroblastoma cell line priority journal protein expression SH SY5Y neuroblastoma cell line TUNEL assay upregulation Western blotting wound healing cell division cell motion cell survival drug effects G1 phase cell cycle checkpoint gene expression regulation pathology tumor cell line tumor invasion Antineoplastic Agents Antioxidants Cell Division Cell Line, Tumor Cell Movement Cell Proliferation Cell Survival G1 Phase Cell Cycle Checkpoints Gene Expression Regulation, Neoplastic Humans In Situ Nick-End Labeling Iridoids Neoplasm Invasiveness Wound Healing |
Publisher: | Elsevier B.V. | Abstract: | Neuroblastoma is one of the most common types of pediatric tumors that can spread quickly in neuronal tissues. Oleuropein which is active compound of olive leaves, belongs to polyphenols group and has antioxidant, anti-microbial, anti-inflammatory, anti-hypertensive and anti-carcinogenic effects. The aim of the study is to determine the therapeutic effects of oleuropein on cell proliferation, invasion, colony formation, cell cycle and apoptotic mechanisms in SH-SY5Y neuroblastoma cell line under in vitro conditions. The effect of oleuropein on cell viability was determined by XTT method. 84 cell cycle control and 84 apoptosis related genes were evaluated by RT-PCR. Effects of oleuropein on apoptosis were researched by TUNEL assay. Protein expressions were determined by western blot analysis. Effects of oleuropein on cell invasion, colony formation and migration were detected by matrigel-chamber, colony formation assay and wound-healing assay, respectively. IC50 value of oleuropein in SH-SY5Y cells was detected as 350 µM at 48th hours. It is determined that oleuropein causes cell cycle arrest by down-regulating of CylinD1,CylinD2,CyclinD3,CDK4,CDK6 and up-regulating of p53 and CDKN2A, CDKN2B, CDKN1A gene expressions. Oleuropein also induces apoptosis by inhibiting of Bcl-2 and activating of Bax,caspase-9 and caspase-3 gene expressions. Apoptotic cell ratio was found 36.4 ± 3.27% in oleuropein dose group. Oleuropein decreased invasion in SH-SY5Y cells and suppressed colony numbers in ratio of 53.6 ± 4.71%.Our results demonstrated that oleuropein can be a therapeutic agent in the treatment of neuroblastoma. © 2016 Elsevier B.V. | URI: | https://hdl.handle.net/11499/9523 https://doi.org/10.1016/j.gene.2016.03.038 |
ISSN: | 0378-1119 |
Appears in Collections: | PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection Tıp Fakültesi Koleksiyonu WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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