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Title: | Ferulic acid decreases cell viability and colony formation while inhibiting migration of MIA PaCa-2 human pancreatic cancer cells in vitro | Authors: | Fahrioğlu, Umut Dodurga, Yavuz Elmas, Levent Seçme, Mücahit |
Keywords: | Apoptotic genes Cell cycle genes Ferulic acid Pancreatic cancer Treatment caspase 10 caspase 3 caspase 8 caspase 9 cyclin D1 cyclin dependent kinase 4 cyclin dependent kinase 6 death receptor 4 death receptor 5 Fas associated death domain protein ferulic acid gelatinase A gelatinase B nicotinamide adenine dinucleotide adenosine diphosphate ribosyltransferase phosphatidylinositol 3,4,5 trisphosphate 3 phosphatase protein Bax protein bcl 2 protein bcl xl protein Bid protein kinase B protein Noxa protein p16 protein p21 protein p53 PUMA protein retinoblastoma protein tissue inhibitor of metalloproteinase 1 tissue inhibitor of metalloproteinase 2 trypan blue tumor necrosis factor receptor associated death domain protein coumaric acid antineoplastic activity apoptosis Article cancer cell culture cell assay cell cycle cell invasion cell viability cell viability assay kit colony formation controlled study cytotoxicity assay gene expression gene expression profiling human human cell human cell culture IC50 in vitro study migration inhibition pancreatic cancer cell line priority journal real time polymerase chain reaction tumor invasion drug effects Neoplasm Metastasis pancreas tumor pathology tumor cell line wound healing Cell Line, Tumor Coumaric Acids Humans Pancreatic Neoplasms Real-Time Polymerase Chain Reaction Wound Healing |
Publisher: | Elsevier | Abstract: | Novel and combinatorial treatment methods are becoming sought after entities in cancer treatment and these treatments are even more valuable for pancreatic cancer. The scientists are always on the lookout for new chemicals to help them in their fight against cancer. In this study, we examine the effects of ferulic acid (FA), a phenolic compound, on gene expression, viability, colony formation and migration/invasion in the cultured MIA PaCa-2 human pancreatic cancer cell. Cytotoxic effects of FA were determined by using trypan blue dye exclusion test and Cell TiterGlo (CTG) assay. IC50 dose in MIA PaCa-2 cells was detected as 500µM/ml at the 72nd hour. Expression profiles of certain cell cycle and apoptosis genes such as CCND1 (cyclin D1),CDK4, CDK6, RB, p21, p16, p53, caspase-3, caspase-9, caspase-8, caspase-10, Bcl-2, BCL-XL,BID, DR4,DR5,FADD,TRADD,PARP, APAF, Bax, Akt, PTEN, PUMA, NOXA, MMP2, MMP9, TIMP1 and TIMP2 were determined by real-time PCR. The effect of FA on cell viability was determined by CellTiter-Glo® Luminescent Cell Viability Assay. Additionally, effects of FA on colony formation and invasion were also investigated. It was observed that FA caused a significant decrease in the expression of CCND1, CDK 4/6, Bcl2 and caspase 8 and 10 in the MIA PaCa-2 cells while causing an increase in the expression of p53, Bax, PTEN caspase 3 and 9. FA was observed to decrease colony formation while inhibiting cell invasion and migration as observed by the BioCoat Matrigel Invasion Chamber guide and colony formation assays. In conclusion, FA is thought to behave as an anti-cancer agent by affecting cell cycle, apoptotic, invasion and colony formation behavior of MIA PaCa-2 cells. Therefore, FA is placed as a strong candidate for further studies aimed at finding a better, more effective treatment approach for pancreatic cancer. © 2015 Elsevier B.V. | URI: | https://hdl.handle.net/11499/9717 https://doi.org/10.1016/j.gene.2015.10.061 |
ISSN: | 0378-1119 |
Appears in Collections: | PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection Tıp Fakültesi Koleksiyonu WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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